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Comparison of different karyotyping methods in filamentous ascomycetes – a case study of Nectria haematococca

Published online by Cambridge University Press:  01 November 1998

M. TAGA
Affiliation:
Department of Biology, Faculty of Science, Okayama University, Tsushima, Okayama 700, Japan
M. MURATA
Affiliation:
Research Institute for Bioresources, Okayama University, Kurashiki 710, Japan
H. SAITO
Affiliation:
Department of Plant Protection, National Agriculture Research Center, Ministry of Agriculture, Forestry, and Fisheries, 3-1-1 Kannondai, Tsukuba, Ibaraki 305, Japan
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Abstract

In some filamentous ascomycetes, karyotypic data by conventional light microscopy are not consistent with the electrophoretic karyotypes by pulsed field gel electrophoresis (PFGE). In this study, the three methods, conventional light microscopy on asci, PFGE, and the germ tube burst method (GTBM), were compared for their concordance in karyotyping by using Nectria haematococca (anamorph: Fusarium solani). GTBM was included in the study for cytological karyotyping on mitotic metaphase in germlings of conidia. Conventional light microscopy on asci was concluded to lead to the underestimation of chromosome number in this fungus. PFGE was effective for analysing chromosomes smaller than ca 6 Mb, but did not yield a complete karyotype due to the poor resolution of larger chromosomes. GTBM was useful for determining chromosome numbers as well as chromosome morphology. Analyses by these methods revealed that each of five strains used, two homothallic and the other three heterothallic, has a unique karyotype different from others in number and size of chromosomes. The present results indicate that combination of PFGE and GTBM constitutes a powerful tool to determine karyotypes of filamentous ascomycetes including N. haematococca.

Type
Research Article
Copyright
© The British Mycological Society 1998

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