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Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

  • Anna M. FITZGERALD (a1), Agnieszka M. MUDGE (a2) (a3), Andrew P. GLEAVE (a2) and Kim M. PLUMMER (a1)

Abstract

We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.

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Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

  • Anna M. FITZGERALD (a1), Agnieszka M. MUDGE (a2) (a3), Andrew P. GLEAVE (a2) and Kim M. PLUMMER (a1)

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