Skip to main content Accessibility help
×
Home

Ultrathin Films of Oriented Bacteriorhodopsin: Nanostructured Films for Investigating the Primary Photoevent in Vision Processes

  • Rigoberto C. Advincula (a1) and Mi-kyoung Park (a1)

Abstract

In this work, a protocol for investigating Bacteriorhodopsin (BR) biomimetic systems as ultrathin films is presented. BR is one of the most well studied proteins important for investigating the primary photo-event in vision processes. The use of macromolecular assembly approaches for deposition onto solid support substrates, e.g. SiOx, gold- or ITO-coated glass (electrode) provide advantages in that surface sensitive measurements can be used to correlate photocurrent generation, photoelectric response, pH change, chromophore behavior, etc. with protein orientation at interfaces. Membrane and protein morphology were correlated to measurements using surface sensitive techniques, such as atomic force microscopy (AFM), ellipsometry, quartz crystal microbalance (QCM), etc. on solid-substrate systems. These studies can lead to applications in optobioelectronic devices (biosensors) including patterning in transducer array configurations, where the film structure is important. Hybrid films are possible with supramolecular assembly approaches, e.g. adsorption of membrane with lipidbilayers. We report our initial results on highly ordered and oriented BR protein arrays of controlled thickness, layer order, and orientation. This was done primarily using the alternate polyelectrolyte deposition (APD) or layer-by-layer (LbL) approach to functionalize substrate surfaces.

Copyright

References

Hide All
1. Ulman, A., An Introduction to Ultrathin Organic Films: From Langmuir Blodgett to Self Assembly, Academic Press: Boston, 1991.
2. (a) Oesterhelt, D., Stoeckenius, W., Nature, New Biol. 149, 233, (1971). (b) W. Stoeckenius, R.A. Bogomolni, Annu. Rev. Biochem., 52, 587 (1982).
3. (a) Birge, R., Annu. Rev. Phys. Chem. 41, 683 (1990) and References cited therein.(b) C. Brauchle, N. Hampp, D. Oesterhelt, Adv. Mater. 3, 420 (1991). (c) D. Oesterhelt, C. Brauchle, N. Hampp, Q. Rev. Biophys. 24, 425 (1991) and References cited therein.
4. (a) Miyasaka, T., Koyama, K. Appl. Opt. 32, 6371 (1993). (b) T. Miyasaka, K. Koyama, I. Otoh, Science 255, 342 (1992).
5. (a) Birge, R.R. Am. Sci. 82, 348 (1994)., (b) M. Wolperdinger, N. Hampp, Biophys. Chem. 56, 189 (1995).
6. Miyasaka, T., Koyama, K. Thin Solid Films 210/211, 146 (1992).
7. Keszthelyi, L., Biochim. Biophys. Acta 598, 429 (1980).
8. Brizzolara, R., BioSystems 35, 137 (1995).
9. Koyama, K., Yamaguchi, N., Miyasaka, T., Science 265, 762 (1994).
10. Koyama, K., Yamaguchi, N., Miyasaka, T., Adv. Mater. 7, 590 (1995).
11. Decher, G., Hong, J., Makromol. Chem. 46, 321 (1992).
12. Decher, G., Science 277, 1232 (1997).
13. Ferreira, F., Rubner, M., Macromolecules 28, 7107 (1995).
14. He, J. A., Samuelson, L., Li, L., Kumar, J., Tripathy, S.K. Langmuir 14, 1674 (1998).
15. Hampp, N., Chem. Rev. 100, 1755 (2000).

Related content

Powered by UNSILO

Ultrathin Films of Oriented Bacteriorhodopsin: Nanostructured Films for Investigating the Primary Photoevent in Vision Processes

  • Rigoberto C. Advincula (a1) and Mi-kyoung Park (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.