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Thermal Properties of Mineralized and Non Mineralized Type I Collagen in Bone

  • L. F. Lozano (a1), M. A. Peña-Rico (a1), H. Jang-Cho (a2), A. Heredia (a1), E. Villarreal (a3), J. Ocotlán-Flores (a4), A. L. Gomez-Cortes (a1), F. J. Aranda-Manteca (a5), E. Orozco (a1) and L. Bucio (a1)...


The research about the structural stability of bone, as a composite material, compromises a complete understanding of the interaction between the mineral and organic phases. The thermal stability of human bone and type I collagen extracted from human bone by different methods was studied in order to understand the interactions between the mineral and organic phases when is affected by a degradation/combustion process. The experimental techniques employed were calorimetry and infrared spectroscopy (FTIR) techniques. The extracted type I collagens result to have a bigger thermal stability with a Tmax at 500 and 530 Celsius degrees compared with the collagen present in bone with Tmax at 350 Celsius degrees. The enthalpy value for the complete degradation/combustion process were similar for all the samples, being 8.4 +- 0.11 kJ/g for recent bones diminishing with the antiquity, while for extracted collagens were 8.9 +- 0.07 and 7.9 +-1.01 kJ/g. These findings demonstrate that the stability loss of type I collagen is due to its interactions with the mineral phase, namely carbonate hydroxyapatite. This cause a change in the molecular properties of the collagen during mineralization, specifically in its cross-links and other chemical interactions, which have a global effect over the fibers elasticity, but gaining tensile strength in bone as a whole tissue. We are applying this characterization to analyze the diagenetic process of bones with archaeological interest in order to identify how the environmental factors affect the molecular structure of type I collagen. In bone samples that proceed from an specific region with the same environmental conditions, the enthalpy value per unit mass was found to diminish exponentially with respect to the bone antiquity.



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1. Collins, M., Riley, M., Child, A. and Turner-Walker, G.. 1995. A basic mathematical simulation of the chemical degradation of ancient collagen. J. Archaeol. Sci 22, 175183.
2. Grupe, G. 1995. Preservation of collagen in bone from dry, sandy soil. J. Archaeol. Sci. 22, 193199.
3. Hedges, E. M., and Millard, A.. 1995. Bones and groundwater: Towards the modelling of diagenetic processes. J. Archaeol. Sci. 22, 155164.
4. Hedges, E. M., and Millard, A.. 1995. Measurements and relationships of diagenetic alteration of bone from three archaeological sites. J. Archaeol. Sci. 22, 201209.
5. Katzenberg, M. and Harrison, R.. 1997. What's in a bone? Recent advances in archaeological bone chemistry. J. Archaeol. Res. Vol. 5, No. 3, pp. 265293.
6. Lozano, L. F., Peña-Rico, M.A., Heredia, A., Ocoltlan-Flores, J., Gomez-Cortes, A.L., Velazquez, R. and Bucio, L. Thermal Analysis Study of Human Bone. 2002. Submitted.
7. Nielsen-Marsh, C., Hedges, R., Mann, T. and Collins, M.. 2000. A preliminary investigation of the application of differential scanning calorimetry to the study of collagen degradation in archaeological bone. Thermochim. Acta. 365, 129139.
8. Schoeninger, M., Moore, K., Murray, M. and Kingston, J.. 1989. Detection of bone preservation in archaeological and fossil samples. Applied Geochemistry, Vol. 4, pp. 281292.
9. Vento, C.E., Rodríguez, SR & Franco, M.L., 1981. La datación Absoluta por el método de colágeno residual en Cuba. Kobie (Bilbao), Grupo espeleológico Vizcaíno. Diputación Federal de Vizcaya. Vol. 11.


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