Skip to main content Accessibility help

TEM Observations of Bio-Conjugated Streptavidin-Gold Nanoparticles

  • Ai Leen Koh (a1) and Robert Sinclair (a2)


Simultaneous visualization of inorganic nanoparticles and their bio-functionalized coating under the TEM can be achieved with negative staining, where electron dense material is introduced onto the specimen to enhance contrast. Using this technique, streptavidin-functionalized gold nanoparticles were negatively stained using phosphotungstic acid (PTA) at pH 7.0 and then observed using the TEM. The proteins appear as light regions/halos surrounding the gold nanoparticles, with widths similar to those of streptavidin protein molecules reported in literature. Experiments were performed to bio-conjugate streptavidin-gold nanoparticles to biotinylated antibodies and biotinylated actin, and then negatively staining the samples with PTA for TEM analyses. In the first experiment, an increase in halo widths in more than 60% of the particles was observed. In the second experiment, the nanoparticles were found to localize around the long actin filaments. Negative staining is useful for studying bio-functionalized nanoparticles as it enables the proteins surrounding the nanoparticles to be imaged and studied using the TEM.



Hide All
1. Thach, R. E. and Thach, S. S., Biophys. J. 1971, 11, 204210 10.1016/S0006-3495(71)86208-2
2. Hayat, M. A. and Miller, S. E. in Negative Staining. (Mc-Graw Hill Publishing Company, 1990) pp. 15.
3. Li, H., Park, S. H., Reif, J. H., LaBean, T. H. and Han, Y., J. Am. Chem. Soc. 2004, 126, 418419 (2004).10.1021/ja0383367
4. Cross, P. C. and Mercer, K. L in Cell and Tissue Ultrastructure – A Functional Perspective. (W. H. Freeman and Company, 1993) page 30.
5. Parak, W. J., Gerion, D., Pellegrino, T., Zanchet, D., Micheel, C., Williams, S. C., Boudreau, R., Gros, M. A Le, Larabell, C. A. and Alivisatos, A. P., Nanotechnology 14 R15– R27 (2003).10.1088/0957-4484/14/7/201


TEM Observations of Bio-Conjugated Streptavidin-Gold Nanoparticles

  • Ai Leen Koh (a1) and Robert Sinclair (a2)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed