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Substrate effect on Dynamic Indentation Measurement of Biological Cell Properties

Published online by Cambridge University Press:  31 January 2011

Guoxin Cao  and
Namas Chandra
Guoxin Cao
Affiliation:
gcao2@unl.edu, Univeristy of Nebraska-Lincoln, Engineering Mechanics, 317 Nebraska Hall, Lincoln, Nebraska, 68588, United States
Namas Chandra
Affiliation:
nchandra2@unlnotes.unl.edu, University of Nebraska-Lincoln, Lincoln, Nebraska, United States
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Abstract

Viscoelastic mechanical properties of biological cells are commonly measured using atomic force microscope (AFM) dynamic indentation method with spherical tips. Storage and loss modulii of cells are then computed from the indentation force-displacement response under dynamic loading conditions. It is shown in current numerical simulations that those modulii computed based on existing analysis can not reflect the true values due to the substrate effect. This effect can alter the indentation modulus by changing the geometric relations between the indentation displacement and the contact area. Typically, the cell modulii are significantly overestimated in the existing indentation analysis.

Keywords

biomaterialviscoelasticitysimulation
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK05-36
Copyright
Copyright © Materials Research Society 2009

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