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Stable, Nanoscale Glycosphingolipid Films for Use in Sensing Applications

Published online by Cambridge University Press:  17 March 2011

Rory Stine ,
Cara-Lynne Schengrund  and
Michael V. Pishko
Rory Stine
Affiliation:
Departments of Chemical Engineering, Chemistry, and Materials Science & Engineering The Pennsylvania State University
Cara-Lynne Schengrund
Affiliation:
Department of Biochemistry and Molecular Biology, Milton Hershey Medical Center, The Pennsylvania State University
Michael V. Pishko*
Affiliation:
Departments of Chemical Engineering, Chemistry, and Materials Science & Engineering The Pennsylvania State University
*
*Author to whom correspondence should be sent: 104 Fenske Laboratory University Park, PA 16802 mpishko@engr.psu.edu
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Abstract

We have developed a means of producing thin, oriented lipid monolayers which are stable under repeated washing and which may be useful in biosensing or surface- coating applications. Phosphotidylcholine (PC) and the glycosphingolipid (GSL) GM1 were used as representative lipids for this process. Initially, a mixed self-assembled monolayer of octanethiol and hexadecanethiol was constructed on a clean gold surface. This hydrophobic surface was then brought into contact with a thin lipid layer that had been deposited at the air/liquid interface of a solution by evaporating a mixture of lipid in hexane on top of a layer of water. The lipid layer, now deposited on the gold surface, was then heated to cause intercalation of the fatty acid and alkanethiol chains, and cooled to form a highly stable film which withstood repeated rinsing and solution exposure. Presence and stability of the film was confirmed via ellipsometry, FTIR, and QCM, with an average overall thickness of ∼3.5 nm. These films may be potentially useful in biotoxin detection or as a protein resistant layer for the prevention of biofouling.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W12.2
Copyright
Copyright © Materials Research Society 2004

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