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Single Cell Based Microelectrode Array Biosensors

  • Mo Yang (a1), Shalini Prasad (a2), Xuan Zhang (a1), Mihrimah Ozkan (a2) (a3) and Cengiz S. Ozkan (a1)...

Abstract

Extracellular potential is an important parameter which indicates the electrical activity of live cells. Membrane excitability in osteoblasts plays a key role in modulating the electrical activity in the presence of chemical agents. The complexity of cell signal makes interpretation of the cellular response to a chemical agent very difficult. By analyzing shifts in the signal power spectrum, it is possible to determine a frequency spectrum also known as Signature Pattern Vectors (SPV) specific to a chemical. It is also essential to characterize single cell sensitivity and response time for specific chemical agents for developing detect-to-warn biosensors. We used a 4x4 multiple Pt microelectrode array to spatially position single osteoblast cells, by using a gradient AC field. Fast Fourier Transformation (FFT) and Wavelet Transformation (WT) analyses were used to extract information pertaining to the frequency of firing from the extracellular potential.

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Single Cell Based Microelectrode Array Biosensors

  • Mo Yang (a1), Shalini Prasad (a2), Xuan Zhang (a1), Mihrimah Ozkan (a2) (a3) and Cengiz S. Ozkan (a1)...

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