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A Silicon Nanopore Device for On-Chip Patch Clamp Measurements of Single Ion Channel Activity

  • L. Plucinski (a1) (a2), Y. Chen (a1) (a2) and G. L. Liu (a1) (a2)

Abstract

Here we present a novel silicon nanopore planar patch clamp chip for single ion channel screening. We fabricate our devices using a combination of KOH and metal-assisted etching. Electrical characterization shows that the shunt capacitance and access resistance are within the accepted ranges for single channel recordings. In order to test our devices, we cultured and differentiated human neuroblastoma SH-SY5Y cells on chip. We reliably obtained a high resistance seal to the cell membrane and report single ion channel activity recordings.

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References

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1. Penner, R., “A Practical Guide to Patch Clamping,” in Single-Channel Recording, 2nd ed. Sakmann, B. and Neher, E. Eds. Springer, 2009, pp. 128.
2. Py, C. et.al. “A novel silicon patch-clamp chip permits high-fidelity recording of ion channel activity from functionally defined neurons,” Biotechnol. and Bioeng., vol. 107, Nov. 2010, pp. 593600.
3. Dunlop, J. et.al. “High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology,” Nat. Rev. Drug Discov., vol. 7, April 2008, pp. 358368.

Keywords

A Silicon Nanopore Device for On-Chip Patch Clamp Measurements of Single Ion Channel Activity

  • L. Plucinski (a1) (a2), Y. Chen (a1) (a2) and G. L. Liu (a1) (a2)

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