Skip to main content Accessibility help
×
Home

Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor

  • Joonhyung Lee (a1), Youn-Suk Choi (a1), Yeolho Lee (a1), Hun Joo Lee (a1), Jung Nam Lee (a1), Sang Kyu Kim (a1), Kyung Yeon Han (a1), Eun Chol Cho (a1), Jae Chan Park (a1) and Soo Suk Lee (a1)...

Abstract

We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a mass-sensitive device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

Copyright

References

Hide All
1. Wu, G. H., Datar, R. H., Hansen, K. M., Thundat, T., Cote, R. J., and Majumdar, A., Nat. Biotechnol. 19, 856 (2001).
2. Lee, J., Icoz, K., Roberts, A., Ellington, A. D., Savran, C. A., Anal. Chem. 82, 197 (2010).
3. Yang, L. T., Fung, C. W., Cho, E. J., and Ellington, A. D., Anal. Chem. 79, 3320 (2007).
4. Savran, C. A., Knudsen, S. M., Ellington, A. D., and Manalis, S. R., Anal. Chem. 76, 3194 (2004).
5. Lyon, L. A., Musick, M. D., and Natan, M. J., Anal. Chem. 70, 5117 (1998).
6. Knudsen, S. M., Lee, J., Ellington, A. D., and Savran, C. A., J. Am. Chem. Soc. 128, 15936 (2006).
7. Lee, H. J., Namkoong, K., Cho, E. C., Ko, C., Park, J. C., Lee, S. S., Biosens. Bioelectron. 24, 3120 (2009).
8. Du, J., Harding, G. L., Ogilvy, J. A., Dencher, P. R., and Lake, M., Sens. Actuators, A: Phys. 56, 211 (1996).
9. Jakoby, B., and Vellekoop, M. J., Sens. Actuators, A: Phys. 68, 275 (1998).
10. Gizeli, E., Goddard, N. J., Lowe, C. R., and Stevenson, A. C., Sens. Actuators, B 6, 131 (1992).
11. Gizeli, E., Stevenson, A. C., Goddard, N. J., and Lowe, C. R., IEEE Trans. Ultason. 39, 657 (1992).
12. Kovacs, G., and Venema, A., Appl. Phys. Lett. 61, 639 (1992).
13. Fu, Y. Q., Luo, J. K., Du, X. Y., Flewitt, A. J., Li, Y., Markx, G. H., Walton, A. J., and Milne, W. I., Sens. Actuators, B 143, 606 (2010).
14. Bender, F., Cernosek, R., and Josse, F., Electron. Lett. 36, 1672 (2000).
15. Kim, D., Daniel, W. L., and Mirkin, C. A., Anal. Chem. 81, 9183 (2009).
16. Kavsak, P. A., MacRae, A. R., Yema, M., and Jeffe, A. S., Clinical Chemistry 55, 573 (2009).
17. Mohammed, M., and Desmulliez, M. Y., Lab Chip 11, 569 (2011).
18. Kondoh, J., Matsui, Y., and Shiokawa, S., Jpn. J. Appl. Phys. 32, 2376 (1993).
19. Masson, J. F., Obando, L., Beaudoin, S., and Booksh, K., Talanta. 62, 865 (2004).
20. Wei, J., Mu, Y., Song, D., Fang, X., Liu, X., Bu, L., Zhang, H., Zhang, G., Ding, J., Wang, W., Jin, Q., and Luo, G., Anal. Biochem. 321, 209 (2003).

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed