Skip to main content Accessibility help
×
Home

Production of Highly Ordered Nanoporous Alumina and its Application in Cell Cultivation

  • Andreas Hoess (a1), Andrea Staeudte (a2), Annika Thormann (a3), Martin Steinhart (a4) and Andreas Heilmann (a5)...

Abstract

Nanoporous alumina membranes were utilized as cell culture substrates. Two different anodization processes were applied in order to obtain highly ordered, self-supporting nanoporous alumina membranes. The resulting membranes exhibit uniform nanopores with constant diameters having aspect ratios (pore depth:pore diameter) larger than 1000. Tentative experiments evaluating the cellular interaction of a hepatoma cell line (HepG2) with the nanoporous alumina membranes will be described. Furthermore, cell proliferation as well as the adhesion behavior of the cells on the porous substrates was investigated. Scanning electron microscopy (SEM) and focused ion beam (FIB) technology were applied to examine the cell morphology. The experiments revealed that the nanoporous membranes can be used as cell culture substrates and that they have no adverse effects on cell proliferation and cell viability. Besides, cells on membranes with pore diameters larger than 200 nm developed small cell extensions (filopodia) which penetrated into the nanopores.

Copyright

References

Hide All
1. Furneaux, R.C., Rigby, W.R. and Davidson, A.P., Nature 337, 147 (1989)
2. Thompson, G.E., Thin Solid Films 297, 192 (1997)
3. Jessensky, O., Müller, F. and Gösele, U., J. Electrochem. Soc. 145, 3735 (1998)
4. Popat, K.C., Swan, E.E.L., Mukhatyar, V., Chatvanichkul, K.-I., Mor, G.K., Grimes, C.A. and Desai, T.A., Biomaterials 26, 4516 (2005)
5. Swan, E.E.L., Popat, K.C., Grimes, C.A. and Desai, T.A., J. Biomed. Mater. Res. A 72, 288 (2005)
6. Briggs, E.P., Walpole, A.R., Wilshaw, P.R., Karlsson, M. and Pålsgård, E., J. Mater. Sci.-Mater. Med. 15, 1021 (2004)
7. Karlsson, M., Johansson, A., Tang, L. and Boman, M., Microsc. Res. Tech. 63, 259 (2004)
8. Thormann, A., Teuscher, N., Pfannmöller, M., Rothe, U. and Heilmann, A., Small 3, 1032 (2007)
9. Masuda, H. and Fukuda, K., Science 268, 1466 (1995)
10. Masuda, H., Yada, K. and Osaka, A., Jpn. J. Appl. Phys. 37, L1340 (1998)

Keywords

Related content

Powered by UNSILO

Production of Highly Ordered Nanoporous Alumina and its Application in Cell Cultivation

  • Andreas Hoess (a1), Andrea Staeudte (a2), Annika Thormann (a3), Martin Steinhart (a4) and Andreas Heilmann (a5)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.