Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-25T14:13:34.923Z Has data issue: false hasContentIssue false
  • English
  • Français

Polymer Surface Modification Causes Change in Phenotypic Expression of Primary Bone Cells

Published online by Cambridge University Press:  15 February 2011

Sean A. F. Peel ,
R. N. Sodhi ,
Tran Minh Duc  and
John E. Davies
Sean A. F. Peel
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
R. N. Sodhi
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
Tran Minh Duc
Affiliation:
ESCA Centre for Surface Nanoanalysis and Technology, University Claude Bernard Lyon 1, Lyon, France.
John E. Davies*
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
*
#Address for correspondence: J.E. Davies, Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
Get access

Abstract

Primary rat bone marrow cells were cultured on bacteriological grade polystyrene dishes which had been treated in selected areas with concentrated sulphuric acid. X-ray photoelectron spectroscopy, angle-resolved and imaging modes, and atomic force microscopy showed that the acid treatment brought about both chemical and topographical changes in the substratum surface. While the bone marrow cells attached to both treated and untreated areas of the surfaces of the dishes, the distribution of early mineralized extracellular matrix in these areas was reproducibily different. Thus, using extracellular matrix deposition as a marker, modification of the polymer surface resulted in the cells adhering to treated and untreated areas exhibiting different phenotypes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 71
Copyright
Copyright © Materials Research Society 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Shelton, R.M., Davies, J.E., and Tarrant, S.F. Abst 3B2–11. Trans 3rd World Biomaterials Congress, Kyoto, (1988)Google Scholar
2. Martin, G.R., and Rubin, H., Exp. Cell Res. 85,319 (1974)CrossRefGoogle Scholar
3. Sodhi, R.N.S., Shelton, R.M. & Davies, J.E., The Behaviour of Primary Bone Cells on Characterized Polystyrene Surfaces, submitted for publication.Google Scholar
4. Carter, S.B., Nature 213, 256 (1967)CrossRefGoogle Scholar
5. Lydon, M. J and Clay, C.S., Cell Biol. Intl. Rep. 2,911 (1985)CrossRefGoogle Scholar
6. Shay, J.W. and Walker, C., Scanning Electron Micrsc. 1980/II, 171 (1980).Google Scholar
7. Shelton, R.M. and Davies, J.E. in The Bone-Biomaterial Interface, edited by J.E., Davies (Toronto Univeristy Press, Toronto, 1991) pp. 181–198.Google Scholar
8. Maniatopoulos, C., Sodek, J. and Melcher, A.H., Cell Tiss Res 254, 317 (1988)CrossRefGoogle Scholar
9. Lee, A., Shelton, R.M., Sodhi, R. and Davies, J.E., presented at the llth Annual Canadian Biomaterials Society Conference, London, ON, 1990 (unpublished)Google Scholar
10. Davies, J.E., Chernecky, R., Lowenberg, B. and Shiga, A., Cells & Materials 1, 3 (1991).Google Scholar