Hostname: page-component-84b7d79bbc-fnpn6 Total loading time: 0 Render date: 2024-07-30T07:33:59.017Z Has data issue: false hasContentIssue false
  • English
  • Français

Osteogenic differentiation of ES cell-derived EBs mediated by embedded BMP-2 and TGF-beta-1 in a polyelectrolyte multilayer film

Published online by Cambridge University Press:  01 February 2011

Mjahed Hajare ,
Cortial Delphine ,
Haikel Youssef ,
Dierich Andree ,
Voegel Jean-Claude  and
Benkirane Jessel nadia
Mjahed Hajare
Affiliation:
INSERM, Biomaterials, INSERM UMR 595, Faculté de médecine, 11 rue Humann,, Strasbourg, 67084, France, 00 33 3 90 24 33 76, 00 33 3 90 24 33 79
Cortial Delphine
Affiliation:
delphine.cortial@medecine.u-strasbg.fr, INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
Haikel Youssef
Affiliation:
Haikel@medecine.u-strasbg.fr, INSERM, Faculté de Chirurgie Dentaire,Université Louis Pasteur, Strasbourg, 67000, France
Dierich Andree
Affiliation:
dirich@igbmc.u-strasbg.fr, CNRS, ES, IGBMC, BP 10142, Strasbourg, France., Strasbourg, 67084, France
Voegel Jean-Claude
Affiliation:
voegel@medecine;u-strasbg.fr, INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
Benkirane Jessel nadia
Affiliation:
nadia.jessel@medecine.u-strasbg.fr, INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
Get access

Abstract

In recent years, considerable effort has been devoted to the design and controlled fabrication of structured materials with functional properties. The layer by layer buildup of polyelectrolyte multilayer films (PEM films) from oppositely charged polyelectrolytes1 offers new opportunities for the preparation of functionalized biomaterial coatings. This technique allows the preparation of supramolecular nano-architectures exhibiting specific properties in terms of control of cell activation and may also play a role in the development of local drug delivery systems. Peptides, proteins or DNA, chemically bound to polyelectrolytes, adsorbed or embedded in PEM films, have been shown to retain their biological activities. Recently, tissue engineering has merged with stem cell technology with interest to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries disorders because of the low self-regenerating capacity of the matrix secreting cells. We present here for the first time that embedded BMP-2 and TGFβ1 in a multilayered polyelectrolyte film can drive embryonic stem cells to the cartilage or bone differentiation depending on supplementary co-factors. We selected a model system made from layer by layer poly-ℓ-glutamic acid (PℓGA) and poly-ℓ-lysine succinylated (PℓLs) films into which BMP-2 and TGFβ1 have been embedded. Our results demonstrate clearly that we are able to induce osteogenesis in embryonic stem cells mediated by growth factors embedded in a polyelectrolyte multilayer film.

Keywords

tissuebiomaterialbone
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D10-04
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. , Oberts, , A.B & Sporn, , , M.B. The transforming growth factor-betas. In Peptide growth factors and their receptors. Edited by: Sporn, MB, Roberts, AB. Springer Verlag, Heidelberg, Germany; 421–472 (1990)Google Scholar
2. Chen, P., Carrington, J.L., Hammonds, R.G. & Reddi, A.H. Stimulation of chondrogenesis in limb bud mesoderm cells by recombinant human bone morphogenetic protein 2B (BMP- 2B) and modulation by transforming growth factor β1 and β2. Exp. Cell. Res.195, 509–515 (1994)Google Scholar
3. Resnick, J.L., Bixler, L.S., Cheng, L & Donovan, P.J. Longterm proliferation of mouse primordial germ cells in culture. Nature. 359, 550–551 (1992)Google Scholar
4. Doetschmann, T.C., Eistetter, H., Katz, M., Schmidt, W. & Kemler, R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol. 87, 27–45 (1985)Google Scholar
5. Brüstle, O. et al. Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science. 285:754–756 (1996)Google Scholar
6. K, Johkura et al. Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants. Cardiovasc. Res. 58, 435–443 (2003)Google Scholar
7. Wobus, A.M., Wallukat, G. & Hescheler, J. Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and CAE channel blockers. Differentiation. 48, 173–182 (1994)Google Scholar
8. Okabe, S., Forsberg-Nilsson, K., Spiro, A.C., Segal, M. & McKay, R.D: Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro. Mech. Dev. 59, 89–102 (1996)Google Scholar
9. Soria, B., Skoudy, A. & Martin, F. From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus. Diabetologia. 44, 407–415 (2001)Google Scholar
10. Dani, C. et al. Differentiation of embryonic stem cells into adipocytes in vitro . J. Cell. Sci 110, 1279–1285 (1997)Google Scholar
11. Zur Nieden, N.I., Kempka, G. & Ahr, H.J. In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation. 71, 18–27 (2003)Google Scholar
12. Phillips, B.W., Belmonte, N., Vernochet, C., Ailhaud, G. & Dani, C. Compactin enhances osteogenesis in murine embryonic stem cells. Biochem. Biophys. Res. Com. 284, 478–484 (2001)Google Scholar
13. Buttery, L.D.K. et al. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue. Engineering. 7, 89–99 (2001)Google Scholar
14. Hegert, C. et al. Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells. J. Cell. Sci. 115, 4617–4628 (2002)Google Scholar
15. Wang, D.Y., Rogach, A.L. & Caruso, F. Semiconductor quantum dot-labeled microsphere bioconjugates prepared by stepwise self-assembly. Nano. Lett. 2, 857–861 (2002).Google Scholar
16. Decher, G. Fuzzy nanoassemblies: Toward layered polymeric multicomposites. Science 277, 1232–1237 (1997).Google Scholar
17. Lvov, Y., Onda, M., Ariga, K. & Kunitake, T. Ultrathin films of charged polysaccharides assembled alternately with linear polyions. J. Biomater. Sci.-Polym. Ed. 9, 345–355 (1998).Google Scholar
18. Serizawa, T., Yamaguchi, M. & Akashi, M. Enzymatic hydrolysis of a layer-by-layer assembly prepared from chitosan and dextran sulfate. Macromolecules 35, 8656–8658 (2002).Google Scholar
19. Sato, K., Suzuki, I. & Anzai, J. Preparation of polyelectrolyte-layered assemblies containing cyclodextrin and their binding properties. Langmuir. 19, 7406–7412 (2003).Google Scholar
20. Berth, G., Voigt, A., Dautzenberg, H., Donath, E. & Mohwald, H. Polyelectrolyte complexes and layer-by-layer capsules from chitosan/chitosan sulfate. Biomacromolecules. 3, 579–590 (2002).Google Scholar
21. Hiller, J., Mendelsohn, J.D. & Rubner, M.F. Reversibly erasable nanoporous antireflection coatings from polyelectrolyte multilayers. Nat. Mater. 1, 59–63 (2002).Google Scholar
22. Jessel, N. et al. Build-up of polypeptide multilayer coatings with anti-inflammatory properties based on the embedding of piroxicam-cyclodextrin complexes. Adv. Funct. Mater. 14, 174–182 (2004).Google Scholar
23. Jessel, N. et al. Bioactive coatings based on a polyelectrolyte multilayer architecture functionalized by embedded proteins. Adv. Mater. 15, 692–695 (2003).Google Scholar
24. Jessel, N. et al. Pyridylamino-beta-cyclodextrin as a molecular chaperone for lipopolysaccharide embedded in a multilayered polyelectrolyte architecture. Adv. Funct. Mater. 14, 963–969 (2004).Google Scholar
25. Jessel, N. et al. Short-time tuning of the biological activity of functionalized polyelectrolyte. Adv. Funct. Mater. 15, 648–654 (2005).Google Scholar
26. Jessel, N. et al. Control of monocyte morphology on and response to model surfaces for implants equipped with anti-inflammatory agents. Adv. Mater. 16, 1507–1511 (2004).Google Scholar
27. Kilsdonk, E.P. et al. Cellular cholesterol efflux mediated by cyclodextrins. J. Biol. Chem. 270, 17250–17256 (1995)Google Scholar
28. Francius, G et al. Effect of crosslinking on the elasticity of polyelectrolyte multilayer films measured by colloidal AFM. Microsc. Res. Tech. 69, 84–92 (2006)Google Scholar
29. Jessel, N. et al. Multiple and time scheduled in situ DNA delivery mediated by β- cyclodextrin embedded in a polyelectrolyte multilayer. Proc. Natl. Acad. Sci. (USA), 103, 8618–8621(2006)Google Scholar
30. Sauerbrey, G. Verwendung von Schwingquartzen zur Wägung dünner Schichten und zur Mikrowägung. Z. Phys. 155, 206–222 (1959).Google Scholar
31. Picart, C. et al. Buildup mechanism for poly(L-lysine)/hyaluronic acid films onto a solid surface. Langmuir. 17, 7414–7424 (2001)Google Scholar
32. Walcz, E. et al. Complete coding sequence, deduced primary structure, chromosomal localization, and structural analysis of murine aggrecan. Genomics. 22, 364–71 (1994)Google Scholar
33. Konecki, D.S. et al. Hypoxanthine guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. Nucl. Acids Res. 10, 6763–6775 (1982)Google Scholar
34. Metsäranta, M., Toman, D., De Crombrugghe, B., Vurio, E. Mouse type II collagen gene. Complete nucleotide sequence, exon structure and alternative splicing. J. Biol. Chem. 266, 16862–16869 (1991)Google Scholar
35. Desbois, C., Hogue, D.A. and Karsenty, G. The mouse osteocalcin gene cluster contains three genes with two separate spatial and temporal patterns of expression. J. Biol. Chem. 269, 1183–1190 (1994)Google Scholar
36. Morinobu, M. et al. Osteopontin expression in osteoblasts and osteocytes during bone formation under mechanical stress. J. Bone. Mine. Res. 18, 1706–1715 (2003).Google Scholar