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Osteoblast - Orthopaedic Biomaterial Response

  • L. Zou (a1), W.R. Walsh (a1) (a2), H. Keeping (a1), C. R. Howlett (a3), J. Steele (a4), C. Mcfarland (a4), M. Russell (a1) and M.G. Ehrlich (a1)...


An understanding of bone cell response and extracellular matrix production to a biomaterial is crucial for development of new prosthetic devices. The nature of the cellular-biomaterial surface interface will depend upon a number of factors including substrate properties (surface chemistry, charge, topography) as well as biological cellular concerns (i.e. adsorption of attachment factors to the surface, growth factors). The quality of the matrix and bone-bonding may be influenced by these factors. Recently, a short-term in-vitro cell culture assay has demonstrated the initial attachment and spread of human derived bone cells on metallic (titanium and stainless steel) and polymeric surfaces to be dependent on the adsorption of adhesive attachment factor proteins (fibronectin and vitronectin) to the substratum surface [1]. The morphological appearance of human osteoblasts cultured on titanium and stainless steel with time also demonstrated differences compared to tissue culture plastic [2]. Little data however, is available for the mitogenic and gene expression levels of primary human bone cells cultured on commonly used orthopaedic materials and the response of these cells to growth factors. The present study examined the mitogenic response and steady state mRNA expression levels of primary human bone cells cultured on metallic substrates to provide further insight into the nature of cell-substrate interactions.



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1. Howlett, C R, Evans, M D M, Walsh, W R, Johnson, G, Steele, J G, The mechanism of initial attachment of cells derived from human bone to commonly used prosthetic materials during cell culture, Biomaterials, (in press).
2. Walsh, W R, Howlett, C R, Zou, L, Russell, M, Keeping, H, Ehrlich, M G, Morphology of osteoblasts during initial attachment to metal and plastic surfaces. Society for Biomaterials 1994 (submitted)
3. Aufmkok, B, Schwartz, E R,. Biochemical characterizations of human osteoblasts in culture. Prog. Clin. Biol. Res. 187:201,1985
4. Whitson, S W, Whitson, M A, Bowers, D E Jr., Falk, M C, Factors influencing synthesis and mineralization of bone matrix from bovine bone cells growth in vitro. J Bone Mineral Res. 7(7):727, 1992
5. Beresford, J N, Gallagher, J, Russell, R G G. 1,25-dihydroxyvitamin D3 and human bone-derived cell in vitro: Effects on alkaline phosphatase, type I collagen and proliferation. Endocrinology 119(4): 1776, 1986
6. Kim, Y J, Sah, R L, Doong, J Y H, Grodzinsky, A J. Fluorometric assay of DNA in cartilage explants using Heochest 33258. Analytic Biochem. 174: 168, 1988
7. Chomczynski, B, Sacchi, N, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156, 1987
8. Keeping, H S, Winters, S J, Attardi, B, Troen, P. Developmental changes in testicular inhibin and andtogen-binding protein during sexual maturation in the cynomolgus monkey, macaca fascicularis. Endocrinology 126: 2858, 1990
9. Villarreal, X C, Mann, K G, Long, G L. Structure of human osteonectin based upon analysis of cDNA and genomic sequences. Biochemistry 28: 6483, 1989
10. Celeste, A J, Rosen, V, Buekner, J L, Kriz, R, Wang, E A, Wozney, J M. Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones. EMBO J. 5: 1885, 1986
11. Max, M, Danie, T O, Kashgarian, M, Madri, J A. Spatial organization of the extracellular matrix modulates the expression of PDGF-receptor subunits in Mesangial cells. Kidney Intl. 43(5): 1027, 1993
12. Stein, G S, Lian, J B, Owen, T A. Relationship of cell growth to the regulation of tissuespecific gene expression during osteoblast differentiation. FASEB. 4: 3111, 1990

Osteoblast - Orthopaedic Biomaterial Response

  • L. Zou (a1), W.R. Walsh (a1) (a2), H. Keeping (a1), C. R. Howlett (a3), J. Steele (a4), C. Mcfarland (a4), M. Russell (a1) and M.G. Ehrlich (a1)...


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