Skip to main content Accessibility help
×
Home

Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

  • James P. Landry (a1), X. D. Zhu (a1), X. W. Guo (a1) and J. P. Gregg (a2)

Abstract

We have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

Copyright

References

Hide All
1. Schena, Mark, Microarray Analysis. (John Wiley and Sons, Hoboken, 2003) pp. 117, p. 184.
2. MacBeath, G. and Schreiber, S. L., Science 289, 1760 (2000).
3. Wong, A. and Zhu, X. D., Appl. Phys. A 63, 1 (1996).
4. Zhu, X. D., Lu, H. B., Guo-Zhen, Yang, Zhi-Yuan, Li, Ben-Yuan, Gu, Dao-Zhong, Zhang, Phys. Rev. B 57, 2514 (1998).
5. Zhu, X. D., Si Weidong, Xi, X. X., Qidu, Jiang, Appl. Phys. Lett. 78, 460 (2001).
6. Schwarzacher, W., Gray, J., Zhu, X. D., Electrochemical and Solid-State Lett. 6, C73 (2003).
7. A, R. M.. Azzam and Bashara, N. M., Ellipsometry and Polarized Light. (Elsevier Science, New York, 1987) p. 305.
8. Gruber, H. J., Hahn, C. D., Kada, G., Riener, C. K., Harms, G. S., Ahrer, W., Dax, T. G., Knaus, H. G., Bioconjugate Chem. 11, 696 (2000).
9. Lemeshko, S. V., Powdrill, T., Belosludtsev, Y. Y., Hogan, M., Nucleic Acids Res. 29, 3051 (2001).
10. Gray, D. E., Case-Green, S. C., Fell, T. S., Dobson, P. J., Southern, E. M., Langmuir 13, 2833 (1997).

Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

  • James P. Landry (a1), X. D. Zhu (a1), X. W. Guo (a1) and J. P. Gregg (a2)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed