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Nano-scale Debye Capacitive Sensors for Highly Sensitive, Label-free, Nucleic Acid Analysis

  • Manu Sebastian Mannoor (a1), Teena James (a2), Dentcho V. Ivanov (a3), Bill Braunlin (a4) and Les Beadling (a5)...

Abstract

An electrochemical capacitive sensor with electrode separation in the order of the Electrical Double Layer width (Debye length) of the analyte solution is presented for extremely sensitive and label-free analysis of Nucleic Acids. As the Electrical Double Layers (EDL) from both the capacitive electrodes interact and overlap each other in the reduced space confinement, the contribution from the electrode polarization effects and noises due to bulk sample resistance are found to be minimized. The dielectric property changes during hybridization reaction were measured using 10-mer nucleotide sequences. A 30-45% change in relative permittivity (capacitance) was observed due to DNA hybridization at 10Hz.

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Keywords

Nano-scale Debye Capacitive Sensors for Highly Sensitive, Label-free, Nucleic Acid Analysis

  • Manu Sebastian Mannoor (a1), Teena James (a2), Dentcho V. Ivanov (a3), Bill Braunlin (a4) and Les Beadling (a5)...

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