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Nanofiber-permeated, hybrid polymer/ceramic scaffolds for guided cell behavior

Published online by Cambridge University Press:  22 August 2014

Clarke Nelson ,
Yusuf Khan  and
Cato T. Laurencin
Clarke Nelson
Affiliation:
Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, Connecticut
Yusuf Khan
Affiliation:
Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, Connecticut Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, Storrs, CT
Cato T. Laurencin
Affiliation:
Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, Connecticut Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, Storrs, CT
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Abstract

The current gold-standard therapeutic strategies for bone grafts in the patient population are to use either allograft or autograft bone. Although these approaches have a long track record of utilization, neither is without risk to the patient, and there remains a desire in the field to improve treatment options. While there have been treatments approved by the FDA for full length growth factors and calcium salt-laden collagen sponges, these are not available for the entire population of potential bone graft patients. One viable strategy to focus on these concerns is to design an implantable bone graft substitute that can address all the negative drawbacks of autograft bone, allograft bone, and full length proteins. The work provides a preliminary investigation of synthetic, nanofiber-permeated, composite polymer/ceramic scaffold for bone repair using thermally induced phase separation, PLLA microspheres, and hydroxyapatite. The scaffolds as described have fiber diameters that mimic natural collagen ECM networks in bone as determined by scanning electron microscopy and will serve as the basis for future studies in substrate-guided bone tissue regeneration.

Keywords

nanoscalebonebiomaterial
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1687: Symposium W – Functional Biomaterials for Regenerative Engineering , 2014 , mrss14-1687-w05-07
Copyright
Copyright © Materials Research Society 2014 

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References

Borden, M., et al. ., Tissue-engineered bone formation in vivo using a novel sintered polymeric microsphere matrix. J Bone Joint Surg Br, 2004. 86(8): p. 1200–8.CrossRefGoogle ScholarPubMed
Calori, G.M., et al. ., The use of bone-graft substitutes in large bone defects: any specific needs? Injury. 42 Suppl 2: p. S56–63.CrossRefGoogle Scholar
Colnot, C., Zhang, X., and Tate, M.L., Current insights on the regenerative potential of the periosteum: Molecular, cellular, and endogenous engineering approaches. J Orthop Res.Google Scholar
Lee, N.K., et al. ., Endocrine regulation of energy metabolism by the skeleton. Cell, 2007. 130(3): p. 456–69.CrossRefGoogle ScholarPubMed
Borden, M., et al. ., Tissue engineered microsphere-based matrices for bone repair: design and evaluation. Biomaterials, 2002. 23(2): p. 551–9.CrossRefGoogle ScholarPubMed
Ulery, B.D., Nair, L.S., and Laurencin, C.T., Biomedical Applications of Biodegradable Polymers. J Polym Sci B Polym Phys, 2011. 49(12): p. 832–864.CrossRefGoogle ScholarPubMed
Khan, Y.M., Katti, D.S., and Laurencin, C.T., Novel polymer-synthesized ceramic composite-based system for bone repair: an in vitro evaluation. J Biomed Mater Res A, 2004. 69(4): p. 728–37.CrossRefGoogle Scholar
Daculsi, G., et al. ., Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics. Calcif Tissue Int, 1990. 46(1): p. 20–7.CrossRefGoogle ScholarPubMed
Murphy, W.L., Kohn, D.H., and Mooney, D.J., Growth of continuous bonelike mineral within porous poly(lactide-co-glycolide) scaffolds in vitro. J Biomed Mater Res, 2000. 50(1): p. 50–8.3.0.CO;2-F>CrossRefGoogle ScholarPubMed
Khan, Y., El-Amin, S.F., and Laurencin, C.T., In vitro and in vivo evaluation of a novel polymer-ceramic composite scaffold for bone tissue engineering. Conf Proc IEEE Eng Med Biol Soc, 2006. 1: p. 529–30.CrossRefGoogle ScholarPubMed
Glowacki, J., Mizuno, S., and Greenberger, J.S., Perfusion enhances functions of bone marrow stromal cells in three-dimensional culture. Cell Transplant, 1998. 7(3): p. 319–26.CrossRefGoogle ScholarPubMed
Rauh, J., et al. ., Bioreactor systems for bone tissue engineering. Tissue Eng Part B Rev. 17(4): p. 263–80.CrossRefGoogle Scholar
Yu, X., et al. ., Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization. Proc Natl Acad Sci U S A, 2004. 101(31): p. 11203–8.CrossRefGoogle ScholarPubMed
Laron, D. and Pandya, N.K., Advances in the orthopedic management of osteogenesis imperfecta. Orthop Clin North Am, 2013. 44(4): p. 565–73.CrossRefGoogle ScholarPubMed
Behring, J., et al. ., Toward guided tissue and bone regeneration: morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review. Odontology, 2008. 96(1): p. 1–11.CrossRefGoogle ScholarPubMed
Muschler, G.F., et al. ., Evaluation of collagen ceramic composite graft materials in a spinal fusion model. Clin Orthop Relat Res, 1996(328): p. 250–60.CrossRefGoogle Scholar
Sverzut, A.T., et al. ., Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses. Biomed Mater. 7(3): p. 035007.CrossRefGoogle Scholar
Takeuchi, Y., Nakayama, K., and Matsumoto, T., Differentiation and cell surface expression of transforming growth factor-beta receptors are regulated by interaction with matrix collagen in murine osteoblastic cells. J Biol Chem, 1996. 271(7): p. 3938–44.CrossRefGoogle ScholarPubMed
Ambrosio, A.M., et al. ., A novel amorphous calcium phosphate polymer ceramic for bone repair: I. Synthesis and characterization. J Biomed Mater Res, 2001. 58(3): p. 295–301.3.0.CO;2-8>CrossRefGoogle ScholarPubMed
Jordan, D.R., et al. ., A synthetic hydroxyapatite implant: the so-called counterfeit implant. Ophthal Plast Reconstr Surg, 1998. 14(4): p. 244–9.CrossRefGoogle ScholarPubMed
Ma, P.X. and Zhang, R., Synthetic nano-scale fibrous extracellular matrix. J Biomed Mater Res, 1999. 46(1): p. 60–72.3.0.CO;2-H>CrossRefGoogle ScholarPubMed
Liu, W., Thomopoulos, S., and Xia, Y., Electrospun nanofibers for regenerative medicine. Adv Healthc Mater, 2012. 1(1): p. 10–25.CrossRefGoogle ScholarPubMed
Brown, J.L., et al. ., Composite scaffolds: bridging nanofiber and microsphere architectures to improve bioactivity of mechanically competent constructs. J Biomed Mater Res A, 2010. 95(4): p. 1150–8.CrossRefGoogle ScholarPubMed
Lakshmi, S., Katti, D.S., and Laurencin, C.T., Biodegradable polyphosphazenes for drug delivery applications. Adv Drug Deliv Rev, 2003. 55(4): p. 467–82.CrossRefGoogle ScholarPubMed
Cicchinelli, L.D., Gonzalez San Juan, M., and Testa, J. Aycart, Current concepts of absorbable fixation in first ray surgery. Clin Podiatr Med Surg, 1996. 13(3): p. 533–47.Google ScholarPubMed
El-Amin, S.F., et al. ., Integrin expression by human osteoblasts cultured on degradable polymeric materials applicable for tissue engineered bone. J Orthop Res, 2002. 20(1): p. 20–8.CrossRefGoogle ScholarPubMed
Laurencin, C.T. and Khan, Y., Regenerative engineering. Sci Transl Med. 4(160): p. 160ed9.CrossRefGoogle Scholar
Mesfin, A., et al. ., High-dose rhBMP-2 for adults: major and minor complications: a study of 502 spine cases. J Bone Joint Surg Am. 95(17): p. 1546–53.CrossRefGoogle Scholar
Nelson, C., et al. ., Nanostructured Composites for Bone Repair. Journal of Biomaterials and Tissue Engineering, 2013. 4(4): p. 426–439.CrossRefGoogle Scholar
Wei, G. and Ma, P.X., Partially nanofibrous architecture of 3D tissue engineering scaffolds. Biomaterials, 2009. 30(32): p. 6426–34.CrossRefGoogle ScholarPubMed
Gittens, R.A., et al. ., Implant osseointegration and the role of microroughness and nanostructures: Lessons for spine implants. Acta Biomater.Google Scholar
Lu, C.C., et al. .,Complications and technical pitfalls of titanium elastic nail fixation for midclavicular fractures. Orthopedics. 37(4): p. e377–83.CrossRefGoogle Scholar
Attawia, M.A., Devin, J.E., and Laurencin, C.T., Immunofluorescence and confocal laser scanning microscopy studies of osteoblast growth and phenotypic expression in three-dimensional degradable synthetic matrices. J Biomed Mater Res, 1995. 29(7): p. 843–8.CrossRefGoogle ScholarPubMed
Garg, K. and Bowlin, G.L.,Electrospinning jets and nanofibrous structures. Biomicrofluidics. 5(1): p. 13403.CrossRefGoogle Scholar
Robey, P.G., Bone Matrix Proteoglycans and Glycoproteins, in Principles of Bone Biology, Bilezikian, J.P., Raisz, L.G., and Rodan, G.A., Editors. 1996, Academic Press: San Diego, CA. p. 155–165.Google Scholar
Weeks, S., et al. ., The effects of chemokine, adhesion and extracellular matrix molecules on binding of mesenchymal stromal cells to poly(l-lactic acid). Cytotherapy. 14(9): p. 1080–8.CrossRefGoogle Scholar
El-Amin, S.F., et al. ., Extracellular matrix production by human osteoblasts cultured on biodegradable polymers applicable for tissue engineering. Biomaterials, 2003. 24(7): p. 1213–21.CrossRefGoogle ScholarPubMed