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Morphological Changes of Isolated Osteoclasts in Cell Culture Due to Different Biomaterials

Published online by Cambridge University Press:  26 February 2011

J. Thomas Lambrecht ,
R. Ewers ,
A. Kerscher  and
R. Jentzsch
J. Thomas Lambrecht
Affiliation:
Department of Oral and Maxillo-Facial Surgery, Christian-Albrechts-University Kiel (West-Germany)
R. Ewers
Affiliation:
Department of Oral and Maxillo-Facial Surgery, Christian-Albrechts-University Kiel (West-Germany)
A. Kerscher
Affiliation:
Department of Oral and Maxillo-Facial Surgery, Christian-Albrechts-University Kiel (West-Germany)
R. Jentzsch
Affiliation:
Department of Oral and Maxillo-Facial Surgery, Christian-Albrechts-University Kiel (West-Germany)
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Abstract

Osteoclasts have been isolated in primary cell culture using femoral bone of laying hens being fed on an eight day calcium free diet. Placing these cells on the surface of fixed cortico-femoral chicken bone provoked the feature of resorption pits proving that they are able to resorb bone.

After placing osteoclasts on different biomaterials (Aluminumoxide ceramics, teflon, carbon fibre reinforced polysulphone, polymethylmethacrylate, polydioxanone) scanning electron microscopy was performed. Different materials provoke different morphological features of these cells, probably due to functional variations as a response to the changing surfaces. Adhesion was feasible on all the surfaces, uptake of small surface particles was possible and cell fusion took place on most materials suggesting acceptance of the tested biomaterials by the cells.

The results show that morphological changes of isolated osteoclasts in cell culture can be detected due to different functional challenges of the surfaces of different biomaterials.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 110: Symposium M – Biomedical Materials and Devices , 1987 , 317
Copyright
Copyright © Materials Research Society 1988

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