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LDH-DNA Nanohybrids: a complete biophysical characterization

Published online by Cambridge University Press:  01 February 2011

Fabrice Leroux ,
Málha Ben Belkacen ,
Ghislain Guyot ,
Christine Taviot-Guého ,
Philippe Léone ,
Laurent Cario ,
Léa Desigaux  and
Bruno Pitard
Fabrice Leroux
Affiliation:
Laboratoire des Matériaux Inorganiques UMR 6002
Málha Ben Belkacen
Affiliation:
Laboratoire des Matériaux Inorganiques UMR 6002
Ghislain Guyot
Affiliation:
Laboratoire de Photochimie Moléculaire et Macromoléculaire UMR 65051 - Université Blaise Pascal (Clermont-Ferrand II), 24 avenue des Landais, 63177 Aubière cedex, France E-mail: gueho@chimtp.univ-bpclermont.fr
Christine Taviot-Guého
Affiliation:
Laboratoire des Matériaux Inorganiques UMR 6002
Philippe Léone
Affiliation:
Institut des Matériaux Jean Rouxel, 2 rue de la Houssinière, B.P. 32229 44322 Nantes cedex, France
Laurent Cario
Affiliation:
Institut des Matériaux Jean Rouxel, 2 rue de la Houssinière, B.P. 32229 44322 Nantes cedex, France
Léa Desigaux
Affiliation:
Inserm, U533, 1 rue Gaston Veil, BP 53508, 44035 Nantes cedexl, France
Bruno Pitard
Affiliation:
Inserm, U533, 1 rue Gaston Veil, BP 53508, 44035 Nantes cedexl, France
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Abstract

Recently, LDH have been considered as a new kind of gene delivery system [1, 2]. In this work, we report the formation of magnesium-gallium LDH-DNA nanohybrids using the coprecipitation method. This “self assembly” approach enabled the incorporation of long DNA fragments up to 6000–8000 bp. X-ray diffraction analyses indicate a parallel orientation of DNA double helix in the interlamellar space with respect to the hydroxide sheets. The Mg/Ga molar ratio within the hydroxide layers appears to be determined by DNA macromolecules which may interact with charged complexes that form during cation hydrolysis. The presence of DNA macromolecules also inhibits the crystal growth: hydrodynamic diameter measurements revealed homogeneous populations of particles with a mean diameter ranging from 90 to 150 nm, compatible with cell penetration through endocytosis. Concerning the charge surface of this new DNA delivery system, ζ-potential measurements indicate negative values ranging from –20 to - 40 mV which suggest incomplete DNA intercalation. Yet, this small negative surface might be suitable for protecting DNA from extra-cellular degradations without preventing cell penetration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 847: Symposium EE – Organic/Inorganic Hybrid Materials , 2004 , EE9.18
Copyright
Copyright © Materials Research Society 2005

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