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Laser Direct-Write of Embryonic Stem Cells and Cells Encapsulated in Alginate Beads for Engineered Biological Constructs

Published online by Cambridge University Press:  25 April 2012

T.B. Phamduy ,
A.D. Dias ,
N. Abdul Raof ,
N.R. Schiele ,
D.T. Corr ,
Y. Xie  and
D.B. Chrisey
T.B. Phamduy
Affiliation:
Biomedical Engineering Department, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180
A.D. Dias
Affiliation:
Biomedical Engineering Department, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180
N. Abdul Raof
Affiliation:
College of Nanoscale Science and Engineering, University at Albany, State University of New York, 257 Fuller Rd., Albany, NY 12203
N.R. Schiele
Affiliation:
Biomedical Engineering Department, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180
D.T. Corr
Affiliation:
Biomedical Engineering Department, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180
Y. Xie
Affiliation:
College of Nanoscale Science and Engineering, University at Albany, State University of New York, 257 Fuller Rd., Albany, NY 12203
D.B. Chrisey
Affiliation:
Biomedical Engineering Department, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180 Material Science and Engineering, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, NY 12180
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Abstract

The ability to control the deposition of mouse embryonic stem cells (mESCs), and mESCs encapsulated in 200-μm diameter alginate microbeads, into customized patterns has recently been achieved using laser direct-write (LDW). Gelatin-based LDW was utilized to target and reproducibly deposit groups of cells directly onto receiving substrate surfaces. Live/dead staining for cell viability and immunocytochemistry for the pluripotency marker, Oct-4, indicated that transferred mESCs were viable following transfer, and maintained an important embryonic stem cell marker, respectively. LDW was further used to print mESCs encapsulated in hydrogel microbeads into customized patterns on a single-bead basis. Recent efforts have also achieved patterns of discrete co-cultures of mESCs and breast cancer cells in separate hydrogel microbeads. Altogether, we demonstrated the feasibility of LDW to print patterns of mESCs and mESC-microbeads for the biomimetic assembly of engineered cellular constructs and tissue models.

Keywords

laser ablationbiomaterialtissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm06-08
Copyright
Copyright © Materials Research Society 2012

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