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Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

Published online by Cambridge University Press:  31 January 2011

Atsushi Arakaki ,
Fukashi Masuda  and
Tadashi Matsunaga
Atsushi Arakaki
Affiliation:
arakakia@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
Fukashi Masuda
Affiliation:
50006641226@st.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
Tadashi Matsunaga
Affiliation:
tmatsuna@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
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Abstract

Mms6 is a small acidic protein which is tightly bound to magnetite in the bacterium Magnetospirillum magneticum AMB-1. Mms6 has been previously shown to promote iron-binding capacity as well as modulate the size and morphology of magnetic iron oxide crystals in vitro. In this study, we synthesized iron oxide crystals by using a monolayer-modified substrate. A self-assembled monolayer of octadecyltrimethoxysilane was modified on a silicon substrate. Recombinant Mms6 protein was attached to the substrate through the hydrophobic interactions between the protein molecules and the monolayer. The immobilization of protein molecules on the substrate surface was confirmed by fluorescent labeling of these molecules and subsequent fluorescence microscopy. This protein-modified substrate was then used as a template for iron oxide crystal formation in a ferrous solution. Scanning electron microscopy revealed site-specific formation of iron oxide crystals in substrate regions with immobilized proteins. This use of proteins might provide an alternative method for the bottom-up fabrication of nano-sized magnetic particles.

Keywords

biomaterialbiologicalmagnetic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK03-08
Copyright
Copyright © Materials Research Society 2009

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