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Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

  • Yasuharu Ohgoe (a1), Tomoaki Wada (a1), Yasuyuki Shiraishi (a2), Hidekazu Miura (a2), Kenji K. Hirakuri (a3), Akio Funakubo (a1), Tomoyuki Yambe (a2) and Yasuhiro Fukui (a1)...

Abstract

In this study, in order to investigate biocompatibility of nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) film coating segmented polyurethane (SPU) scaffold fiber sheet (a-C:H:N-Scaffold) in in-vitro test, mouse fibroblasts (NIH 3T3) cells were grown on the a-C:H:N-Scaffold. The cell behavior was monitored by time-lapse imaging system. Additionally, the a-C:H:N-Scaffold was implanted at partial aorta descendens of a goat for 35 days. The surface morphology, composition, and wettability of the a-C:H:N-scaffold was estimated by Scanning Electron Microscope (SEM), X-ray photoelectron spectrometer (XPS), and contact angle measurement. In in-vitro test, it was observed that a-C:H:N film coating had a facilitatory effect on cell motility and cell growth. In in-vivo test, it was observed that the a-C:H:N-Scaffold surface was uniformly covered by neointima. The a-C:H:N-Scaffold surface had no thrombus formation as an inflammatory reaction and it was shown that the a-C:H:N film coating had a good blood compatibility. These results suggest that a-C:H:N film coating has good cytocompatibility and blood compatibility and it is a promising approach for improvement of biocompatibility of biomaterial surfaces.

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Keywords

Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

  • Yasuharu Ohgoe (a1), Tomoaki Wada (a1), Yasuyuki Shiraishi (a2), Hidekazu Miura (a2), Kenji K. Hirakuri (a3), Akio Funakubo (a1), Tomoyuki Yambe (a2) and Yasuhiro Fukui (a1)...

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