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Immobilization of Proteins on the Surface of Silanized Hydroxyapatite

  • Akira Monkawa (a1), Toshiyuki Ikoma (a1) (a2), Yuri Kumagai (a1) (a2), Syunji Yunoki (a1) and Junzo Tanaka (a1) (a2)...

Abstract

Hydroxyapatite (HAp) is widely used as bioceramics for bone and dental tissue reconstructions due to its excellent biocompatibility with hard tissues and high osteoconductivity. Although it is well known that HAp has the high adsorbed ability, its ability is decreased in high ionic solution. To immobilize protein on the HAp surface, this study demonstrates that Collagen (Col) and fibronectin (Fnt) were immobilized on the surface of hydroxyapatite (HAp) sintered body with and without the silane coupling agent of aminopropyltriethoxysilane (APS). The proteins immobilized on the HAp and APS/HAp surfaces were investigated by atomic force microprobe (AFM, Simadzu; SPM 9500) analyses and Ζ potential measurements. The stability of protein/APS/HAp and protein/HAp composites was evaluated after immersion in phosphate buffer and NaCl solutions with various concentrations. AFM analyses and Ζ potential measurements revealed that proteins immobilized on the APS/HAp are more stable than those immobilized on the HAp in high ionic solutions.

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[1] Dupraz, A. M. P., Wijn, J.R. de Meer, S.A. v.d. and Groot, K. de. Characterization of silan e-treated hydroxyapatite powders for use as filler in biodegradable composites. Journal of Biomedical Materials Research, vol. 30, 231238 (1996).
[2] Furuzono, T., Kishida, A., Tanaka, J.. Related. Nano-scaled hydroxyapatite/polymer composite I. Coating of sintered hydroxyapatite particles on poly(gamma-methacryloxypropyl trimethoxysilane)grafted silk fibroin fibers through chemical bonding. Journal Material Science Material Madison. (2004) 15, 1923.
[3] Ikoma, T., Yamazaki, A., Nakamura, S. and Akao, M., “Preparation and Dielectric Property of Sintered Monoclinic Hydroxyapatite”, Journal of Material Science Letters, 18 (1999) 12251228.

Immobilization of Proteins on the Surface of Silanized Hydroxyapatite

  • Akira Monkawa (a1), Toshiyuki Ikoma (a1) (a2), Yuri Kumagai (a1) (a2), Syunji Yunoki (a1) and Junzo Tanaka (a1) (a2)...

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