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Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

  • Praveen Singh (a1), Takeshi Onodera (a2), Kiyoshi Matsumoto (a3), Norio Miura (a4) and Kiyoshi Toko (a5)...

Abstract

A nano-scale biosensor chip surface was fabricated using dinitro-phenylated key hole limpet (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self assembled monolayer (SAM) and bis sulfosuccinimidyl suberate(BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml(140 ppt) for TNT molecule. The detection limit of surface plasmon resonance(SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format. A short injection (12 sec) of 10 mM Glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

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1. Cumming, C.J., Aker, C., Fisher, M., Fox, M., Grone, M.J.L., Reust, D., Rockly, M.G., Swager, T.M., Towers, E. and Williams, V., IEEE Trans. Geosc. Remote Sensing 39, 1119(2001).
2. Miura, N., Higobashi, H., Sakai, G., Takeyasu, A., Uda, T. and Yamazoe, N., Sens. Actuators B 13–14, 188(1993).
3. Yang, J.S. and Swager, T.M., J. Amer. Chem. Soc. 120, 11864(1998).
4. Thundat, T., SPIE's oemagzine February, 2526(2005).
5. Rabbany, S.Y., Lane, W.J., Marganski, W.A., Kusterbeck, A.W. and Ligler, F.S., 2002. J. Immunol. Methods 246, 69(2002)
6. Naimushin, A.N., Soelberg, S.D., Nguyen, D.K., Dunlap, L., Barthollomew, D., Elkind, J., Melendez, J. and Furlong, C., 2002. Biosens. Bioelectron. 17, 573(2002).
7. Chinowsky, T. M., Quinn, J.G., Bartholomew, D.U., Kaiser, R., Elkind, J.L., 2003. Performance of the Spreeta 2000 intergrated surface plasmon resonance affinity sensor. Sens. Actuators B 6954, 19.
8. Homola, J., Yee, S. and Myszka, D., “Surface Plasmon Resonance Biosensors”, Ed. Ligler, S., Taitt, C.A.R., (Elsevier, 2002) The Netherlands, pp. 207251.
9. Shankaran, D. Ravi, Gobi, K.V., Matsumoto, K., Imato, T., Toko, K. and Miura, N., 2004. Sens. Actuators B 100, 450(2004).
10. Matsumoto, K., Torimaru, A., Ishitobi, S., Sakai, T., Ishikawa, H., Toko, K., Miura, N. and Imato, T., Talanta 68, 305(2005).
11. Mark, S.S., Sandhyarani, N., Zhu, C., Campagnolo, C. and Batt, C.A., Langmuir 20, 6808(2004).

Keywords

Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

  • Praveen Singh (a1), Takeshi Onodera (a2), Kiyoshi Matsumoto (a3), Norio Miura (a4) and Kiyoshi Toko (a5)...

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