Skip to main content Accessibility help
×
Home

Heme Proteins Encapsulated in Sol-Gel Derived Silica Glasses and their Reaction with Ligands

  • E. H. Lan (a1), M. S. Davidson (a1), L. M. Ellerby (a2), B. Dunn (a2), J. Selverstone Valentine (a2) and J. I. Zink (a2)...

Abstract

Hemoglobin (Hb) and myoglobin (Mb) were encapsulated in transparent silica glasses prepared by the sol-gel method. The preparation of the silica glasses was tailored so that when proteins were entrapped in the pores of the inorganic matrix, they retained their biochemical activity, i.e. they could bind ligands reversibly. Using optical spectroscopy to monitor ligand binding, we studied the binding of O2, CO, and NO with these two heme proteins encapsulated in silica glasses and compared them to heme proteins in aqueous buffer. Both Hb and Mb in the sol-gel glass bound O2, CO, and NO, producing the same spectroscopic properties as those in aqueous buffer. In addition, silica encapsulated Mb was used to evaluate the rate of ligand (O2) transport through the pores of the glass. When varying oxygen concentration and measuring the time required for full conversion of deoxyMb to MbO2 in the silica gel, the time vs. concentration data followed an exponential trend, as expected for diffusion controlled processes.

Copyright

References

Hide All
1. Antonini, E. and Brunori, M., Hemoglobin and Myoglobin in Their Reaction with Ligands.. (North-Holland Publishing Co., Amsterdam, 1971).
2. Dolphin, D., ed., The Porphyrins, Vol. III, (Academic Press, New York, 1978).
3. Ellerby, L. M., Nishida, C. R., Nishida, F., Yamanaka, S. A., Dunn, B., Valentine, J. S., and Zink, J. I., Science 255, 1113 (1992).
4. Yamanaka, S. A., Nishida, F., Ellerby, L. M., Nishida, C. R., Dunn, B., Valentine, J. S., and Zink, J. I., Chem. Mater. 4, 495 (1992).
5. Avnir, D., Braun, S., and Ottolenghi, M., Supermolecular Architecture in Two and Three Dimensions, Bein, T., ed., ACS Symp. Series 499 (American Chemical Society, New York, 1992).
6. Glezer, V. and Lev, O., J. Am. Chem. Soc. 115,2533 (1993).
7. Audebert, P., Demaille, C., and Sanchez, C., Chem. Mater. 5,911 (1993).
8. Hench, L. L. and West, J. K., Chem. Rev. 90, 33 (1990).
9. Brinker, C. J. and Scherer, G., Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing, (Academic Press, New York, 1990).
10. Wittenberg, J. B., Noble, R. W., Wittenberg, B. A., Antonini, E., Brunori, M., and Wyman, J., J. Biol. Chem. 242, 626 (1967).
11. Chien, J. C., J. Am. Chem. Soc. 91, 2166 (1969).
12. Lougovois, V. and Houston, T. W., Food Chemistry 32, 47 (1989).
13. Wade, R. S. and Castro, C. S., Chem. Res. Toxicol. 3,289 (1990).
14. Lange's Handbook of Chemistry, Dean, J. A., ed., (McGraw Hill, New York, 1985).

Related content

Powered by UNSILO

Heme Proteins Encapsulated in Sol-Gel Derived Silica Glasses and their Reaction with Ligands

  • E. H. Lan (a1), M. S. Davidson (a1), L. M. Ellerby (a2), B. Dunn (a2), J. Selverstone Valentine (a2) and J. I. Zink (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.