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Grating Coupled Surface Plasmon Resonance Enhanced Fluorescence and Its Application for Cell Observation
Published online by Cambridge University Press: 31 January 2011
Abstract
One-dimensional grating with 400 nm pitch was fabricated on a SiO2 glass surface. The grating was applied for detection of fluorescence excited by the electric field of the grating-coupled surface plasmon resonance and highly sensitive observation of fluorescence image by optical microscope. 40-times of fluorescence enhancement was obtained under optimal condition after our systematically investigating depth and duty ratio dependent behavior.
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- Copyright © Materials Research Society 2010
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