Skip to main content Accessibility help
×
Home

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes

  • Seong Yoon Lim (a1), Jae Hong Kim (a1), Joon Seok Lee (a1) and Chan Beum Park (a1)

Abstract

We present a versatile and facile route for highly sensitive detection of analytes through coupling the enlargement of gold nanoparticles (Au NPs) with fluorescence decrease. The fluorescence intensity of dye molecules (e.g., fluorescein or rhodamine B) significantly decreased with the increasing concentration of reducing agents, such as hydrogen peroxide and hydroquinone. The sensitivity for the detection of reducing agents was much higher than other detection methods based on the absorbance measurement of enlarged gold nanoparticles or quantum dot-enzyme hybridization. We could successfully detect acetylthiocholine with the detection limit of several nM orders, using an enzymatic reaction by acetylcholinesterase, a key route for the detection of toxic organophosphate compounds. The fluorescence decreasing approach described in this work requires only a simple addition of fluorescence dye to the reaction solution without any chemical modification. The strategy of fluorescence decrease coupled with nanoparticle growth will be applied on the fluorescent substrate to develop detection templates for highly sensitive optical biosensor.

Copyright

References

Hide All
1. Zayats, M.; Baron, R.; Popov, I.; Willner, I. Nano Lett. 2005, 5, 2125.10.1021/nl048547p
2. Jagetia, G. C.; Menon, K. S. L.; Jain, V. Toxicol. Lett. 2001, 121, 1520.10.1016/S0378-4274(00)00290-3
3. Dutta, K.; Bhattacharyay, D.; Mukherjee, A.; Setford, S. J.; Turner, A. P. F.; Sarkar, P. Ecotox. Environ. Safe. 2008, 69, 556561.10.1016/j.ecoenv.2007.01.004
4. Bültzingslöwen, C.; McEvoy, A. K.; McDonagh, C.; MacCraith, B. D. Anal. Chim. Acta 2003, 480, 275283.10.1016/S0003-2670(02)01653-7
5. Pickup, J. C.; Hussain, F.; Evans, N. D.; Rolinski, O. J.; Birch, D. J. S. Biosens. Bioelectron. 2005, 20, 25552565.10.1016/j.bios.2004.10.002
6. Griffin, J.; Singh, A. K.; Senapati, D.; Rhodes, P.; Mitchell, K.; Robinson, B.; Yu, E.; Ray, P. C. Chem. Eur. J. 2009, 15, 342351.10.1002/chem.200801812
7. Lee, S.; Cha, E.-J.; Park, K.; Lee, S.-Y.; Hong, J.-K.; Sun, I.-C.; Kim, S. Y.; Choi, K.; Kwon, I. C.; Kim, K. et al. Angew. Chem. Int. Ed. 2008, 47, 28042807.10.1002/anie.200705240
8. Maxwell, D. J.; Taylor, J. R.; Nie, S. J. Am. Chem. Soc. 2002, 124, 96069612.10.1021/ja025814p
9. Zhu, L.; Lee, C. S.; DeVoe. D. L. Lab Chip 2006, 6, 115120.10.1039/B511766F
10. Yuan, J.; Guo, W.; Wang, E. Anal. Chem. 2008, 80, 11411145.10.1021/ac0713048

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed