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Formation of Silk Monolayers

  • Wayne S. Muller (a1), Lynne A. Samuelson (a1), Stephen A. Fossey (a1) and David L. Kaplan (a1)


Cast silk membranes exhibit useful properties. However, there is limited control over the molecular architecture in these structures. The Langmuir-Blodgett technique can enhance the control of the membrane structure and allow improved control over membrane properties. We have formed natural silk monolayers using the Langmuir technique. Silk fibroin, regenerated from Bombvx mori cocoons, formed stable monolayers evident from pressure/area isotherms. Multilayers of the silk fibroin monolayers were deposited on a number of substrates and characterized. Transmission Electron Microscopy (TEM) and ellipsometry data provide basic information about the physical characteristics of the monolayer. Preliminary analysis of electron diffraction data of the monolayer indicate polycrystalline structure, consistent with the known structure of silk. Infrared spectrometric analysis of the monolayer using Attenuated Total Reflectance (ATR) gave wavenumbers for Amide I, II, III and V bands which compare with the silk II conformation reported for cast silk membranes.



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1. Kuzuhara, A., Asakura, T., Tomoda, R., Matsunaga, T., J. Biotechnology 5, 199 (1987).
2. Asakura, T., Yoshimizu, H., Kuzuhara, A., Matsunaga, T. J., J. Seric. Sci. Jpn. 57, 203 (1988).
3. Demura, M., Asakura, T., Biotechnol. Bioeng. 33, 598 (1989).
4. Demura, M., Asakura, T., Kurso, T., Biosensors 4, 361 (1989).
5. Demura, M., Asakura, T., Nakamura, E., Tamura, H., Journal of Biotechnol. 10, 113 (1989).
6. Grasset, L., Cordier, D., Ville, A., Process Biochem. 14, 2 (1979).
7. Asakura, T., Kanetake, J., Demura, M., Poly-Plast. Technol. Eng. 28, 453 (1989).
8. Yoshimizu, H., Asakura, T., Journal of Applied Polymer Science 40, 127 (1990).
9. Magoshi, J., Magoshi, Y., Nakamura, S., Journal of Applied Polymer Science, Appl. Polym. Symp. 41, 187 (1985).
10. Magoshi, J., Kamiyama, S., Nakamura, S., Proceedings of the 7th International Wool Textile Research Conference, Tokyo 1, 337 (1985).
11. Kaplan, D.L., Lombardi, S.J., Muller, W.S., Fossey, S., Biomaterials Novel Materials from Biological Sources, edited by Byrom, D. (Stockton Press, New York, 1991), p. 1.
12. Lucas, F., Shaw, J.T.B., Smith, S.G., Adv. Prot. Chem. 13, 107 (1958).
13. Lucas, F., Nature 210, 952 (1966).
14. Minoura, N., Tsukada, M., Masanobu, N., Biomaterials 11, 430 (1990).
15. Calvert, P.D., Encyclopedia Materials Science and Engineering. Biological Macromolecules, (Pergamon Press, Oxford, 1988) p. 334.
16. Zemlin, J.C., Technical Report 69–29-CM (AD684333), US Army Natick Laboratories, Natick MA 1968.
17. Swart, R.M., Langmuir-Blodgett Films, edited by Roberts, G. (Plenum Press, New York, 1990) p. 273.
18. Marsh, R.E., Corey, R.B., Pauling, L., Biochim. Biophysics Acta 16, 1 (1955).
19. Fraser, R.D.B., MacRae, T.R., Conformation of Fibrous Proteins (Academic Press, New York, 1973).
20. Kaplan, D.L., Fossey, S., Viney, C., and Muller, W. in Hierarchically Structured Materials, edited by Aksay, I.A., Eric, Baer, Sarikaya, M., and Tirrell, D. (Mater. Res. Soc. Symp. Proc. 255, Pittsburgh, PA, 1992) pp. 1930.
21. Yoshimizu, H., Asakura, T., J. Appl. Poly. Sci. 40, 1745 (1990).
22. Asakura, T., Kuzuhara, A., Tabeta, R., Saito, H., Macromolecules 18, 1841 (1985).
23. Minoura, N., Masuhiro, T., Masanobu, N., Polymer 31, 265 (1990).

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Formation of Silk Monolayers

  • Wayne S. Muller (a1), Lynne A. Samuelson (a1), Stephen A. Fossey (a1) and David L. Kaplan (a1)


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