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Fabrication and Application of Protein Crystal Microarrays

Published online by Cambridge University Press:  11 February 2011

Yoichiroh Hosokawa ,
Satoshi Matsumura ,
Hiroshi Y. Yoshikawa ,
Hiroshi Masuhara ,
Ryosuke Nakamura ,
Yasuo Kanematsu ,
Keiko Ikeda ,
Ai Shimo-oka  and
Hajime Mori
Yoichiroh Hosokawa
Affiliation:
Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Satoshi Matsumura
Affiliation:
Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Hiroshi Y. Yoshikawa
Affiliation:
Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Hiroshi Masuhara*
Affiliation:
Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Ryosuke Nakamura
Affiliation:
Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Yasuo Kanematsu
Affiliation:
Venture Business Laboratory, Osaka University, Suita, Osaka 565–0871, Japan
Keiko Ikeda
Affiliation:
Protein Crystal Corporation, Honmachibashi-nishi Building, 1–1–3, Honmachi, Chuo-ku, Osaka 541–0053, Japan
Ai Shimo-oka
Affiliation:
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606–8585
Hajime Mori
Affiliation:
Protein Crystal Corporation, Honmachibashi-nishi Building, 1–1–3, Honmachi, Chuo-ku, Osaka 541–0053, Japan Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606–8585
*
* Tel: +81–6–6879–7837, Fax: +81–6–6876–8580 e-mail: masuhara@ap.eng.osaka-u.ac.jp
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Abstract

With the aim of fabricating highly integrated protein microarrays, functional proteins were occluded into a protein crystal, consisting of a proteinaceous occlusion body termed polyhedrin, and the crystals were individually fixed onto a polymer film by using laser trapping and bonding techniques. Individual crystals were trapped by the 1064 nm beam of a Nd3+:YAG laser and placed to the film. The bonding to the film was achieved by irradiating with the single shot of the 800 nm beam of a femtosecond Ti:sapphire laser. Enhanced green fluorescent protein (EGFP) was occluded into the polyhedrin crystal and the crystals were patterned and observed by florescence imaging and single crystal florescence spectroscopy. From these experiments, the biological activity of the bonded crystal was confirmed and their potential as element in protein microarrays was discussed.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 735: Symposium C – Bioinspired Nanoscale Hybrid Systems , 2002 , C3.5
Copyright
Copyright © Materials Research Society 2003

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References

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