Skip to main content Accessibility help

Experimental study of filling carbon nanotubes with nucleic acids

  • Daxiang Cui (a1), Cengiz S. Ozkan (a2), Yong Kong (a1) and Huajian Gao (a1)


Encapsulation of DNA molecules inside carbon nanotubes (CNT) in water were performed under the conditions of 400K and 3Bar. Double stranded DNAs of 2kb and 400bp in length, and single stranded oligonucleotides of 60bp in length were selected as target molecules. Au and Pt nanoparticles and fluorescent dye Cy3 were used as tags. Agarose gel electrophoresis was used to remove DNA molecules attached on the outside walls of CNTs. Laser confocal microscopy, scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM), energy dispersive x-ray spectroscopy (EDX) were employed to confirm the encapsulation process. The results demonstrated that DNA molecules attached to the outside of CNTs can be removed by electrophoresis. Confocal microscopy and HR-TEM observations as well as EDX analysis confirmed that the Cy3-labelled DNA molecules, Au-labelled oligonucleotides and Pt-labelled DNA fragments can indeed be encapsulated inside CNTs. These experimental results support our earlier molecular dynamics simulations on encapsulating oligonucleotides inside CNTs. The DNA-CNT hybrids could be further explored for potential applications in bio-nanotechnology.



Hide All
1. Cui, D., and Gao, H., Biotechnol. Prog. 19, 683 (2003).
2. Niemeyer, C. M., Angew.Chem.Int.Ed. 40, 4128 (2001).
3. Martin, C.R., Kohli, P.. Nature Reviews. 2, 29 (2002).
4. Smith, B. W., Monthioux, M., Luzzi, D. E., Nature 396, 323 (1998).
5. Hirahara, K., Suenaga, K., Bandow, S., Kato, H., Okazaki, T., Shinohara, H., and Iijima, S., Phys. Rev. Lett. 85, 5384 (2000).
6. Hummer, G., Rasalah, J. C., Noworyta, J. P., Nature 414, 188 (2001).
7. Gogotsi, Y., Libera, J. A., Guvenc-Yazicioglu, A., and Megaridis, C. M., Appl. Phys. Lett. 79, 1021 (2001).
8. Tsang, S. C., Davis, J. J., Green, M. L. H., Hill, H. A. O., Leung, Y. C. and Sadler, P. J., J. Chem. Soc. Chem. Comm. 1803 (1995).
9. Saito, R., Dresselhaus, G., and Dressehaus, M. S., Physical Properties of Carbon Nanotubes, Imperial College Press, London 1998.
10. Reed, M. A., Molecular Electronics, Academic Press, London 2000.
11. Bonard, J.M., Weiss, N., Kind, H., ckli, T. Stö, Forro, L., Kern, K., and Chatelain, A., Adv. Mater. 13, 184 (2001).
12. Kumar, A., Pattarkine, M., Bhadbhade, M., Mandale, A B, Ganesh, K. N., Datar, S. S., Dharmadhikari, C. V., and Sastry, M., Adv. Mater. 13, 341–3 (2001).
13. Sastry, M., Kumar, A., Datar, S., Dharmadhikari, C. V., and Ganesh, K. N., Appl. Phys. Lett. 78, 2943 (2001).
14. Petach, H. H., Henderson, W., and Olsen, G. M., J. Chem. Soc. Chem. Commun. 2181 (1994).
15. Mertig, M., Ciacchi, L. C., Seidel, R., Pompe, W., and Vita, A. De, Nano. Lett. 2, 841 (2002).
16. Sambrook, J., Fritsch, E. F., and Maniatis, T., Molecular Cloning: A laboratory manual 2 nd ed Cold Spring Harbor Laboratory Press, USA.
17. Jones, D., New Scientist 3, 145 (1966).
18. Heath, J.R., O'Brien, S.C., Zhang, Q., Liu, Y., Curl, R.F., Kroto, H.W., Tittel, F.K., Smalley, R.E., J. Amer. Chem. Soc. 109, 359 (1985).
19. Majetich, S.A., Artman, J.O., McHenry, M.E., Nuhfer, N.T., Staley, S.W., Phys. Rev. B 48, 845 (1993).
20. Seraphin, S., Zhou, D., Jiao, J., J. Appl. Phys. 80, 1 (1996).
21. Saito, Y., in : Ebbesen, T.W. (Ed.), Carbon nanotubes: Preparation and Properties, CRC press, 1996.
22. Saito, Y., Carbon 33, 979 (1995).
23. Dravid, V.P., Host, J.J., Teng, M.H., Elliott, B.R., Hwang, J.H., Johnson, D.L., Mason, T.O. and Weertman, J.R., Nature 374, 602 (1995).
24. Ugarte, D., Chem. Phys. Lett. 209, 99 (1993).
25. Tsang, S.C., Harris, P.J.F., Green, M.L.H., Nature 362, 520 (1994).
26. Gao, H., Kong, Y., Cui, D., Ozkan, C. S., Nano. Lett. 3, 471 (2003).
27. Cui, D., Tian, F., Kong, Y., Igor, T., and Gao, H., Nanotechnology 15, 154 (2004)
28. Guo, Z., Sadler, P. J., Tsang, S. C., Adv. Mater. 10, 701 (1998).
29. Seeman, N.C., Trends Biotech. 17, 437 (1999).
30. Hua, Q., He, R.Q., Biochim Biophys Acta. 205, 1645 (2003).
31. Dujardin, E., Ebbesen, T.W., Hiura, H., Tanigaki, K., Science 265, 1850(1994).
32. Ugarte, D., Châtelain, A., Heer, W.A. de, Science 274,1897(1997).

Related content

Powered by UNSILO

Experimental study of filling carbon nanotubes with nucleic acids

  • Daxiang Cui (a1), Cengiz S. Ozkan (a2), Yong Kong (a1) and Huajian Gao (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.