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Evaporation Kinetics in the Hanging Drop Method of Protein Crystal Growth

Published online by Cambridge University Press:  26 February 2011

James K. Baird ,
Richard W. Frieden ,
E. J. Meehan ,
Pamela J. Twigg ,
Sandra B. Howard  and
William A. Fowlis
James K. Baird
Affiliation:
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899
Richard W. Frieden
Affiliation:
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899
E. J. Meehan
Affiliation:
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899
Pamela J. Twigg
Affiliation:
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899
Sandra B. Howard
Affiliation:
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899
William A. Fowlis
Affiliation:
National Aeronautics and Space Administration, Space Science Laboratory, Marshall Space Flight Center, AL 35812
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Abstract

We present an engineering analysis of the rate of evaporation of solvent in the hanging drop method of protein crystal growth. Our results are applied to 18 different drop and well arrangements commonly encountered in the laboratory. We take into account the chemical nature of the salt, the drop size and shape, the drop concentration, the well size, the well concentration, and the temperature. We find that the rate of evaporation increases with temperature, drop size, and with the salt concentration difference between the drop and the well. The evaporation possesses no unique half-life. Once the salt in the drop achieves about 80% of its final concentration, further evaporation suffers from the law of diminishing returns.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 87: Symposium O – Materials Processing in the Reduced Gravity Environment of Space , 1986 , 231
Copyright
Copyright © Materials Research Society 1987

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References

1. Blundel, T. L. and Johnson, L. N., Protein Crystallography, (Academic Press, New York, 1976).Google Scholar
2. McPherson, A., Preparation and Analysis of Protein Crystals, (John Wiley, New York, 1982).Google Scholar
3. DeLucas, L. J., et al., J. Crystal Growth 76, 681 (1986).Google Scholar
4. Rosenberger, F., J. Crystal Growth 76, 618 (1986).Google Scholar
5. Baird, J. K., Meehan, E. J. Jr., Xidis, A. L., and Howard, S. B., J. Crystal Growth 76, 694 (1986).Google Scholar
6. Aaronson, H. I. and Lee, J. K., in Lectures on the Theory of Phase Transformations, edited by Aaronson, H. I., (American Institute of Mining, Metallurgical and Petroleum Engineers, New York, 1975) pp. 83–115.Google Scholar
7. (a) Lifshitz, I. M. and Slyozov, V. V., J. Phys. Chem. Solids 19, (1961). (b) J. K. Baird, L. K. Lee, O. O. Frazier, and R. J. Naumann, Theory of Ostwald Ripening in a Two Component System, NASA TM-86564, Marshall Space Flight Center, AL, September 1986. Copies obtainable from the first author or from National Technical Information Service, Springfield, VA 22151.Google Scholar
8. Fowlis, W. A., Baird, J. K., Freeden, R. W., Meehan, E. J. Jr., and Twigg, P., J. Crystal Growth (to be submitted).Google Scholar
9. Handbook of Chemistry and Physics, edited by Weast, R. C. and Astle, M. J., 59th ed. (CRC Press, West Palm Beach, FL, 1978) p. E–1.Google Scholar
10. Guggenheim, E. A. and Stokes, R. H., Equilibrium Properties of Aqueous Electrolyte Solutions, (Pergamon Press, London, 1969). Expand Eq. (2.34) on p. 14 in a series appropriate to the dilute limit.Google Scholar
11. Glasstone, S., Textbook of Physical Chemistry, 2nd ed. (O. Van Nostrand Company, Inc., New York, 1946) p. 483.Google Scholar
12. Reference 9. p. D–269.Google Scholar
13. Daniels, F., et al., Experimental Physical Chemistry, 5th ed. (McGraw-Hill,New York, 1956) p. 86.Google Scholar
14. Cussler, E. L., Diffusion: Mass Transfer in Fluid Systems, (Cambridge University Press, London, 1984) p.106.Google Scholar
15. Reference 9. p. D–232.Google Scholar