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Enhancement of On-chip Bioassay Efficiency With Electrothermal Effect

Published online by Cambridge University Press:  01 February 2011

K. R. Huang ,
J. S. Chang ,
Sheng Der Chao  and
K. C. Wu
K. R. Huang
Affiliation:
sdchao@ntu.edu.tw, NTU, Taipei, Taiwan, Province of China
J. S. Chang
Affiliation:
jschang@iam.ntu.edu.tw, NTU, Taipei, Taiwan, Province of China
Sheng Der Chao
Affiliation:
sdchao@spring.iam.ntu.edu.tw, Institute of Applied Mechanics, Taipei, Taiwan, Province of China
K. C. Wu
Affiliation:
wukc@iam.ntu.edu.tw, NTU, Taipei, Taiwan, Province of China
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Abstract

We have performed the finite element simulations to study the binding reaction kinetics of the analyte-ligand protein pairs, C-reactive protein (CRP) and anti-CRP, in a reaction chamber of a biosensor. For diffusion limited reactions, diffusion boundary layers often develop on the reaction surface, thus hindering the reaction. To enhance the efficiency of a biosensor, a non-uniform AC electric field is applied to induce the electrothermal force which stirs the flow field. Biosensors with different arrangements of the electrode pairs and the reaction surface are designed to study the effects of geometric configurations on the binding efficiency. The maximum initial slope of the binding curve can be 6.94 times of the field-free value in the association phase, under an AC field of 15 rms and an operating frequency of 100 kHz. With the electrothermal effect, it is possible to use a slower flow and save much sample consumption without sacrificing the performance of a biosensor. Several design factors not studied in our previous works such as the thermal boundary conditions are discussed.

Keywords

kineticsthermoelectricsimulation
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1272: Symposia KK/LL/NN/OO/PP – Integrated Miniaturized Materials-From Self-Assembly to Device Integration , 2010 , 1272-KK03-05
Copyright
Copyright © Materials Research Society 2010

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References

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