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DNA Bonding to CVD Diamond Probed by Scanning Electron-, Fluorescence-, and Atomic force- Microscopy

Published online by Cambridge University Press:  01 February 2011

Christoph E. Nebel ,
Hiroshi Uetsuka ,
Buhuslav Rezek ,
Dongchan Shin ,
Norio Tokuda  and
Takako Nakamura
Christoph E. Nebel
Affiliation:
christoph.nebel@aist.go.jp, Diamond Research Center, AIST, Central 2, 1-1-1, Tsukuba, 305-8568, Japan, 0298614836, 0298612773
Hiroshi Uetsuka
Affiliation:
hiroshi.uetsuka@aist.go.jp, Diamond Research Center, AIST, Central 2, 1-1-1, Tsukuba, 305-8568, Japan
Buhuslav Rezek
Affiliation:
rezek@fzu.cz, Institute of Physics, Cukrovarnicka 10,, Praha, 162 53, Czech Republic
Dongchan Shin
Affiliation:
dongchan.shin@aist.go.jp, Diamond Research Center, AIST, Central 2, 1-1-1, Tsukuba, 305-8568, Japan
Norio Tokuda
Affiliation:
n-tokuda@aist.go.jp, Nanotechnology Research Institute, AIST, Central 2-13, 1-1-1, Tsukuba, 305-8568, Japan
Takako Nakamura
Affiliation:
takako-nakamura@aist.go.jp, Center for Advanced Carbon Material, AIST, Central 2, Tsukuba, 305-8568, Japan
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Abstract

Double stranded desoxyribonucleic acid (ds-DNA) layers, bonded to hydrogen terminated polycrystalline diamond, are characterized by scanning electron (SEM), fluorescence (FM), and atomic force microscopy (AFM). DNA grafting has been achieved using photochemical bonding of ω-unsaturated 10-amino-dec-1-ene molecules. SEM detects local variations of electron affinities on polycrystalline diamond, revealing distinct grain structures. FM applied on fluorescence labeled ds-DNA show laterally varying intensities of typically 20 %, which resembles also grain structure as detected by SEM. Contact and tapping mode AFM characterization reveal a tilted DNA bonding to diamond, dense layer formation which gives rise to smoothening of surface properties. The lateral density variation of DNA is attributed to local variations of the photo-electron emission efficiency which affects the photochemical attachment chemistry of amine linker molecules to diamond.

Keywords

biomaterialoptical propertiesdiamond
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 956: Symposium J – Diamond Electronics—Fundamentals to Applications , 2006 , 0956-J09-09
Copyright
Copyright © Materials Research Society 2007

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