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Direct immobilization of DNA on partially functionalized diamond surface

  • Jung-Hoon Yang (a1), Kwang-Soup Song (a2), Shouma Kuga (a3) and Hiroshi Kawarada (a4)

Abstract

Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropatterned diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

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