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Differences in Nanoscale Elasticity of Planar and Nanofibrillar Tissue Cultures

Published online by Cambridge University Press:  23 April 2012

Volkan Mujdat Tiryaki ,
Virginia M. Ayres ,
Ijaz Ahmed  and
David I. Shreiber
Volkan Mujdat Tiryaki
Affiliation:
Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States.
Virginia M. Ayres
Affiliation:
Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States.
Ijaz Ahmed
Affiliation:
Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States.
David I. Shreiber
Affiliation:
Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States.
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Abstract

Astrocytes are cellular bridges between the neurons and capillaries in the blood brain barrier. It was recently suggested that the nanophysical properties of the basement membrane of the blood brain barrier can influence astrocyte and neuron responses. In this work, cerebral cortical astrocytes were cultured on standard poly-L-Lysine coated glass substrates, Aclar substrates, and electrospun polyamide nanofibers whose properties may recapitulate those of the basement membrane. The nanoscale elasticity of each culture environment was investigated by force curve analysis and compared. The elasticity of the individual nanofibers on nanofibrillar surfaces was also investigated. Finally, variations in elasticity of scaffolds were correlated with astrocyte responses.

Keywords

elastic propertiesbiomaterialscanning probe microscopy (SPM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1417: Symposium KK – Biomaterials for Tissue Regeneration , 2012 , mrsf11-1417-kk06-25
Copyright
Copyright © Materials Research Society 2012

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References

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