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Development of Quantum dot Reporters for Immunoassay Applications

Published online by Cambridge University Press:  21 March 2011

D. M. Speckman ,
T. L. Jennings ,
S. D. LaLumondiere  and
S. C. Moss
D. M. Speckman
Affiliation:
The Aerospace Corporation, P. O. Box 92957, Los Angeles, CA 90009
T. L. Jennings
Affiliation:
The Aerospace Corporation, P. O. Box 92957, Los Angeles, CA 90009
S. D. LaLumondiere
Affiliation:
The Aerospace Corporation, P. O. Box 92957, Los Angeles, CA 90009
S. C. Moss
Affiliation:
The Aerospace Corporation, P. O. Box 92957, Los Angeles, CA 90009
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Abstract

Multi-pathogen biosensors that take advantage of sandwich immunoassay detection schemes usually rely on spatial resolution patterns of capture antibodies on a detector plate for pathogen recognition, and typically utilize fluorescent, organic dyes as optical labels to identify captured pathogens. An immunoassay-based, fiber optic detector that utilizes varying sized fluorescent semiconductor quantum dots (QDs) as the reporter labels for different antibodies would have the ability to detect multiple pathogens within a single fiber, using a single excitation source. Such a detector requires that QDs be attached to antibody proteins, such that the specificity of the antibody is maintained. We have been involved in efforts to develop a reproducible method for attaching QDs to antibodies for use in such a biosensor.

We synthesized CdSe/ZnS core-shell QDs of differing size, functionalized their surfaces with several types of organic groups for water solubility, and covalently attached these functionalized QDs to rabbit anti-ovalbumin antibody protein. We also demonstrated that these labeled antibodies exhibit selective binding to ovalbumin antigen. We characterized the QDs at each step in the overall synthesis by UV-VIS absorption spectroscopy and by picosecond (psec) transient photoluminescence (TPL) spectroscopy. TPL spectroscopy measurements indicate that QD lifetime depends on the size of the QD, the intensity of their optical excitation, and whether or not they are functionalized and conjugated to antibody. We describe details of these experiments and discuss the impact of our results on our biosensor program.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 676: Symposium Y – Synthesis, Functional Properties and Applications of Nanostructures , 2001 , Y3.6
Copyright
Copyright © Materials Research Society 2001

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References

REFERENCES

1. Bruchez, M. Jr, Morrone, M., Gin, P., Weiss, S., and Alivisatos, A. P., Science 281, 2013 (1998).Google Scholar
2. Chan, W. C. W. and Nie, S., Science 281, 2016 (1998).Google Scholar
3. Mitchell, G. P., Mirkin, C. A., and Letsinger, R. L., J. Am. Chem. Soc. 121, 8122 (1999).Google Scholar
4. Mattoussi, H., Mauro, J. M., Goldman, E. R., Anderson, G. P., Sundar, V. C., Mikulec, F. V., and Bawendi, M. G., J. Am. Chem. Soc. 122, 12142 (2000).Google Scholar
5. Hines, M. A. and Guyot-Sionnest, P., J. Phys. Chem. 100, 468 (1996).Google Scholar
6. Hermanson, G. T., “Bioconjugate Techniques“ Academic Press, 1996, p. 60.Google Scholar
7. Murray, C. B., Norris, D. J., and Bawendi, M. G., J. Am. Chem. Soc. 115, 8706 (1993).Google Scholar
8. Klimov, V. I., Mikhailovsky, A. A., McBranch, D. W., Leatherdale, C. A., and Bawendi, M. G., Phys. Rev. B 61, R13349 (2000).Google Scholar