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Development of a Collagen-Gag Copolymer Implant for the Study of Tendon Regeneration

Published online by Cambridge University Press:  15 February 2011

L. K. Louie ,
I. V. Yannas  and
M. Spector
L. K. Louie
Affiliation:
Massachusetts Institute of Technology, Cambridge, MA 02139
I. V. Yannas
Affiliation:
Massachusetts Institute of Technology, Cambridge, MA 02139 Brockton/West Roxbury VA Medical Center, West Roxbury, MA 02132
M. Spector
Affiliation:
Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 Brockton/West Roxbury VA Medical Center, West Roxbury, MA 02132
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Abstract

Following injuries resulting in a gap, tendons generally do not heal sufficiently because the defect does not fill with reparative tissue, or the resulting scar tissue is not functionally adequate. The objective of this study was to engineer a collagen-GAG (CG) copolymer to facilitate tendon regeneration. Previous work in our laboratory using similar porous resorbable analogs of extracellular matrix have led to regeneration of skin and peripheral nerve.

Matrices with controlled pore structure were produced by adapting the manufacturing technique developed in our laboratory for grafts with cylindrical geometry. CG suspensions contained within silicone elastomer tubes were frozen and lyophilized in a controlled manner. Quantitative optical microscopy was used to determine the percent porosity, average pore size, and pore orientation of the matrices.

The CG matrices, formed in silicone elastomer tubes, 3.8 mm in diameter, had average pore sizes ranging from 20 to 150 μm. his range of pore sizes was comparable to that obtained previously using a diameter of 1.5 mm.1 The average pore size did not appear to be strongly dependent on the tubing diameter. The homogeneity of the pore structure and the pore channel orientation could be controlled by adjusting the temperature of the bath used for freezing the CG suspension and the velocity with which the graft was immersed into the coolant bath.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 19
Copyright
Copyright © Materials Research Society 1994

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References

1. Loree, H., A Freeze-Drying Process for Fabrication of Polymeric Bridges for Peripheral Nerve Regeneration, Massachusetts Institute of Technology Masters Thesis (1988).Google Scholar
2. Alexander, H., Parsons, J. R., Strauchler, I. D., Corcoran, S. F., Gona, O. and Mayott, C. W., Orthop. Rev., 10, 41 (1981).Google Scholar
3. Davis, P. A., Huang, S. J., Ambrosio, L., Ronnca, D. and Nicolais, L., J. Mat. Sci.:Mat. in Med., 3, 359 (1991).Google Scholar
4. Kato, Y. P., Dunn, M. G., Zawadsky, J. P., Tria, A. J. and Silver, F. H., J. Bone Jt. Surg., 73–A, 561 (1991).Google Scholar
5. Yannas, I. V., Angew. Chem. Int. Ed. Eng., 29, 20 (1990).Google Scholar
6. Stone, K. R., Rodkey, W. R., Webber, R. J., McKinney, L. and Steadman, J. R., Clin. Orthop., 252, 129 (1990).Google Scholar
7. Yannas, I. V., Lee, E., Orgill, D. P., Skrabut, E. M. and Murphy, G. F., Proc. Nat'l. Acad. Sci., USA 86, 933 (1989).Google Scholar
8. Chang, A. S., Yannas, I. V., Krarup, C., Sethi, R., Norregaard, T. V. and Zervas, N. T., Proc. ACS Div. of Polymeric Materials: Sci. and Engr., 59, 906 (1988).Google Scholar