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Conductimetric Detection of Protein and Cancer Cells with Oxide Nanosensors

Published online by Cambridge University Press:  01 February 2011

Janagama Goud ,
P. Markondeya Raj ,
Jin Liu ,
Mahadevan Iyer ,
Z. L. Wang  and
Rao Tummala
Janagama Goud
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Electrical and Computer Engineering, 813 Ferst Drive NW, Atlanta, GA, 30329, United States, 404 894 2652
P. Markondeya Raj
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Jin Liu
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Mahadevan Iyer
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Z. L. Wang
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Rao Tummala
Affiliation:
raj@ece.gatech.edu, Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
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Abstract

Semiconducting oxides are widely known and commercially applied for their gas sensing properties. However, biochemical sensing has mostly depended on optical and electrochemical techniques that are more cumbersome. This work investigates the biosensing characteristics of ZnO nanobelts and ZnO thin films. Zinc oxide thin film sensors showed changes in conductivity after protein functionalization with rabbit IgG and hybridization with anti-rabbit IgG. Conductivity changes were also measured after coating the oxides with MCF-7 cancer cells and its antibodies. In another set of experiments, ZnO nanobelts showed systematic conductivity changes with rabbit IgG protein hybridization. The experimental results in this paper indicate that the conductimetric properties of nano and thin film oxides can be sensitized to protein and cancer cell hybridization reactions. This technique can also be applied to certain other pathogen proteins or toxic proteins from the environment leading to low-cost miniaturized wireless biosensors.

Keywords

nanostructuresemiconductingsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1010: Symposium V – Functional Materials for Chemical and Biochemical Sensors , 2007 , 1010-V06-09
Copyright
Copyright © Materials Research Society 2007

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