Skip to main content Accessibility help

Computer-assisted designing and biofabrication of 3-D hydrogel structures towards thick 3-D tissue engineering

  • Makoto Nakamura (a1), Kenichi Arai (a1), Hideki Toda (a1), Shintaroh Iwanaga (a1), Kozo Ito (a1), Capi Genci (a1) and Toshio Nikaido (a1)...


Engineering and manufacturing of thick and bio-functional tissue products is one of the big issues in tissue engineering. To produce such tissues, we need some innovative technologies, which enable us to build up thick, three-dimensional structures and to arrange multiple types of cells to make complicated tissue structures. Based on such considerations, we have developed a custom-made inkjet 3D bioprinter, which realized both of direct cell printing and 3D laminating printing with cells and hydrogel. Recently, it has been improved, and here we report recent progresses and our achievements with new version 3D bioprinter.

Image based printing mode and active Z-axis control system were added. As a useful structure, an image of multi-honeycomb pattern was designed in computer and next it was copied and finally in total 100 image data were prepared. Using those digital data, 3D image of thick multi-honeycomb structure was reconstructed in computer, and then, laminating printing was carried out using our new version 3D bioprinter with alginate hydrogel. The new version printer showed good performance of 3D laminating printing and finally complicated 3D multi-honeycomb hydrogel structures could be successfully fabricated. It is indicated that fabrication of cell containing 3D structures based on the computer aided designs is feasible and that such biofabrication technologies must contribute to further innovative advancement of tissue engineering.



Hide All
[1] Nakamura, M. Reconstruction of biological three- dimensional tissues: Bioprinting and biofabrication using inkjet technology, in Cell and Organ Printing, Ringeisen, BR, Spargo, BJ, Wu, P eds, Springer, 2010. pp.2334.
[2] Nakamura, M, Iwanaga, S, Arai, K, Toda, H, Capi, G, Nikaido, T. Computer-Assisted Biofabrication:The challenges on manufacturing 3-D biological tissues for tissue and organ engineering. Proceeding of Symposium on VLSI Technology at Kyoto 2011.06.14 .
[3] Sun, W, Welcome to Biofabrication. Biofabrication 1, 010201, 2009.
[4] Mironov, V, Trusk, T, Kasyanov, V, Little, S, Swaja, R, Markwald, R. Biofabrication: a 21st century manufacturing paradigm, Biofabrication 1(2), 022001–, 2009.
[5] Guillemot, F, Mironov, V, Nakamura, M, Bioprinting is coming of age:, Biofabrication 2, 010201–. 2010.
[6] Nakamura, M, Kobayashi, A, Takagi, F, Watanabe, A, Hiruma, Y, Ohuchi, K, Iwasaki, Y, Horie, M, Morita, I, Takatani, S. Biocompatible inkjet printing technique for designed seeding of individual living cells Tissue Eng. 11, 1658–, 2005
[7] Nishiyama, Y, Nakamura, M, Henmi, C, Yamaguchi, K, Mochizuki, S, Nakagawa, H, Takiura, K. Development of three-dimensional bio-printer: Construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology. J Biomech Eng 131(3): 035001–, 2009.
[8] Nishiyama, Y, Nakamura, M, Henmi, C, Yamaguchi, K, Mochizuki, S, Nakagawa, H, Takiura, K. Fabrication of 3D cell supporting structures with multi-materials using the bio-printer. Proc. MSEC 200731064, 2007
[9] Henmi, C, Nakamura, M, Nishiyama, Y, Yamaguchi, K, Mochizuki, S, Takiura, K, Nakagawa, H. New approaches for tissue engineering: three dimensional cell patterning using inkjet technology. Inflammation and Regeneration 28(1), 3640, 2008.
[10] Calvert, P, Printing cells. Science; 318(5848): 208–, 2007.
[11] Nakamura, M, Iwanaga, S, Henmi, C, Arai, K, Nishiyama, Y, Biomatrices and biomaterials for future developments of bioprinting and biofabrication Biofabrication 2 014110–, 2010.
[12] Arai, K, Iwanaga, S, Toda, H, Genci, C, Nishiyama, Y, Nakamura, M, Three-dimensional inkjet biofabrication based on designed images. Biofabrication 3(3):034113–, 2011.



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed