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Comparison of Proliferation and Growth of Human Keratinocytes on Plasma CO-Polymers of Acrylic acid/1,7- Octadiene and Self Assembled Monolayers

  • D. B. Haddow (a1), R. M. France (a1), R. D. Short (a1), S. Macneil (a2) and R. A. Dawson (a2)...

Abstract

Human keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.

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1. Rennekampff, H.O., Kiessig, V. and Hansbrough, J.F., “Current concepts in the development of cultured skin replacements,” J. Surgical Res., 62(2), 288295 (1996).
2. Cooper, E., Wiggs, R., Hutt, D. A., Parker, L., Legget, G. J. and Parker, T. L., “Rates of attachment of fibroblasts to self-assembled monolayers formed by adsorption of alkylthiols onto gold surfaces,” J. Mater. Chem., 7(3), 435441 (1997).
3. Griesser, H. J., Chatelier, R. C., Gengenbach, T. R., Johnson, G. and Steele, J. G., “Growth of human cells on plasma-polymers-putative role of amine and amide groups,” J. Biomat. Sci., Polym. Ed., 5(6), 531554 (1994).
4. Cooper, E., Parker, L., Leggett, G. J. and Parker, T.L., “Cellular interactions with selfassembled monolayers,” Abstracts ofpapers of the Am. Chem. Soc., 213(1), 330 (1997).
5. Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Artre, S. and Allara, D.L., “Endothelial cell growth and protein adsorption on terminally functionalised selfassembled monolayers of alkanethiolates on gold,” Langmuir, 13(13), 34043413 (1997).
6. Goberdhan, N. J., Edgecombe, M., Freedlander, E. and MacNeil, S., “Extracellularmatrix proteins induce changes in intracellular calcium and cyclic AMP signalling systems in cultured human keratinocytes,” Burns, 23(2), 122130 (1997).
7. Rao, J. and Otto, W. R., “Fluorometric DNA assay for cell-growth estimation,” Analyt. Biochem., 207(1), 186192 (1992).
8. France, R.M., Short, R.D., Dawson, R.A. and MacNeil, S., “Attachment of human keratinocytes to plasma co-polymers of acrylic acid/1,7-octadiene and allyl amine/1,7 octadiene,” J. Mater. Chem, 8(1), 3742 (1998).
9. Mrksich, I.M. and Whitesides, G.M., “Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells,” Annual Review of Biophysics and Biomolecular Structure, 25, 5578 (1996).

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Comparison of Proliferation and Growth of Human Keratinocytes on Plasma CO-Polymers of Acrylic acid/1,7- Octadiene and Self Assembled Monolayers

  • D. B. Haddow (a1), R. M. France (a1), R. D. Short (a1), S. Macneil (a2) and R. A. Dawson (a2)...

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