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Characterization of Chemically and Topographically Modified Siloxane Elastomer for Controlled Cell Growth

Published online by Cambridge University Press:  17 March 2011

Amy L. Gibson ,
Leslie H. Wilson ,
Wade R. Wilkerson ,
Adam W. Feinberg ,
Charles A. Seegert ,
Ronald H. Baney  and
Anthony B. Brennan
Amy L. Gibson
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Leslie H. Wilson
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Wade R. Wilkerson
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Adam W. Feinberg
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Charles A. Seegert
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Ronald H. Baney
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
Anthony B. Brennan
Affiliation:
Department of Materials Science and Engineering, Biomedical Engineering Program University of Florida, Gainesville, Fl 32611, USA
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Abstract

A main limitation of biomedical devices is the inability to start, stop, and control cell growth making it crucial to develop biomaterial surfaces that induce a desired cellular response. Micropatterns of ridges and pillars were created in a siloxane elastomer (Dow Corning) by casting against epoxy replicates of a micromachined silicon wafer. Silicone oils were incorporated to determine the change in modulus and surface energy caused by these additives. SEM and white light interference profilometry verified that the micropatterning process produced high fidelity, low defect micropatterns. Mechanical analysis indicated that varying the viscosity, weight percent and functionality of the added silicone oil could change the elastic modulus by over an order of magnitude (0.1-2.3 MPa). As a self-wetting resin, silicone oils migrate to the surface, hence changing the surface properties from the bulk. Both topographical and chemical features define the surface energy, which in combination with elastic modulus, dictate biological activity. The results imply that the morphology, mechanical properties and surface energy of the siloxane elastomer can be modified to elicit a specific cell response as a function of engineered topographical and chemical functionalization.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 711: Symposia FF/GG/HH – Advanced Biomaterials--Characterization, Tissue Engineering and Complexity , 2001 , GG1.4.1
Copyright
Copyright © Materials Research Society 2002

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References

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