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Bio-Hybrid Materials for Immunoassay-Based Sensing

  • Jonathan Fang (a1), Esther H. Lan (a2), Jing C. Zhou (a3) and Bruce Dunn (a4)


The process of encapsulating antibodies in sol-gel was used for sensing various hormones, specifically cortisol, insulin, and C-peptide. A sol-gel optical biosensor for cortisol has been developed for monitoring of crew health on-orbit during space missions. Our studies involving silica sol-gel materials with competitive immunoassays demonstrated linear calibration for cortisol in the range of 2-60 μg/dL, which covers the physiological range of cortisol blood concentration for an adult (2-28 μg/dL). The method of standard additions was used to analyze human serum samples sent to us from a NASA laboratory. Our sol-gel immunosensor values were typically within 20% of the values obtained by NASA-JSC using traditional immuno-binding techniques, with some values having less than a 5% error. Initial results are presented for sensing the hormones insulin and C-peptide.



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1 Dave, B.C., Dunn, B., Valentine, J.S., Zink, J.I., Anal. Chem. 66, A1120 (1994)
2 Lan, E.H, Dunn, B., Zink, J.I., Chem. Mater. 12, 1874 (2000)
3 Li, C.-I., Lin, Y.-H., Shih, C.-L., Tsaur, J.-P., Chau, L.-K., Biosensors and Bioelectronics 17, 323 (2002)
4 Doong, R.-A., Tsai, H.-C., Analytica Chimica Acta 434, 239 (2001)
5 Okada, R., Yamamoto, K., Koda, A., et al., General and Comparative Endocrinology 135, 42 (2004)
6 Morinelli, T.A., Meier, G.P., Webb, J.G., et al., International Immunopharmacology 2, 1995 (2002)
7 Niu, P.D., Molecular and Cellular Endocrinology 162, 131 (2000)
8 Lewis, J.G., Elder, P.A., Journal of Steroid Biochemistry 2, 673 (1985)
9 Lewis, J.G., Manley, L., Townsend, J.C., Elder, P.A., Clinica Chimica Acta 159, 205 (1986)
10 Dunn, B., Miller, J.M., Dave, B.C., Valentine, J.S., Zink, J.I., Acta mater 46, 737 (1998)
11 Livage, J., Beteille, F., Roux, C., Chatry, M., Davidson, P., Acta mater 46, 743 (1998)
12 Livage, J., Sanchez, C., Henry, M., Doeuff, S., Solid State Ionics 32–33, 633 (1989)
13 Tortora, G., Grabowski, S.R., Principles of Anatomy & Physiology, 10th ed. (John Wiley & Sons, Inc., New York, 2003) p. 611612
14 Borchers, A.T., Keen, C.L., Gershwin, M.E., Nutrition 18, 889 (2002)
15 Zhou, J.C., Chuang, M.H., Lan, E.H., Dunn, B., Gillman, P.L., Smith, S.M., J. Mater. Chem. 14, 2311 (2004)
16 Hori, Y., Gu, X., Xie, X., Kim, S.K., PLoS Medicine 2, 347 (2005)
17 Lumelsky, N., Blondel, O., Laeng, P., Velasco, I., Ravin, R., McKay, R., Science 292, 1389 (2001)
18 Hund, E., Massart, D.L., Smeyers-Verbeke, J., Journal of Pharmaceutical and Biomedical Analysis 21, 23 (1999)
19 Li, H., Analytical Letters 24, 473 (1991)
20 Yperman, J., Backer, A.D., Vos, A., Franco, D., Mullens, J., Poucke, L.C. Van, Analytica Chimica Acta 511 (1993)


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Bio-Hybrid Materials for Immunoassay-Based Sensing

  • Jonathan Fang (a1), Esther H. Lan (a2), Jing C. Zhou (a3) and Bruce Dunn (a4)


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