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Biocompatibility, Bioactivity and Mechanical Properties of Portland Cement and Portland Cement-Metakaolin Blends for Bone Tissue Engineering Applications

  • Daniel Gallego (a1), Natalia Higuita (a2), Felipe Garcia (a3), Olga M. Posada (a4), Luis E. Lopez (a5), Alan S. Litsky (a6) and Derek J. Hansford (a7)...


We studied the potential applications of Portland cement and Portland cement-Metakaolin blends as scaffolding materials for load bearing bone tissue engineering. Cementitious pastes were prepared by mixing Portland cement and Metakaolin at different ratios (100:0, 85:15), and hydrated under a concentrated CO2 atmosphere (carbonated pastes). Pastes fabricated similarly, but hydrated under normal atmospheric conditions were used for comparison (non-carbonated pastes). Compressive tests were run to evaluate the mechanical properties of the pastes. The bioactivity of the samples was tested in a simulated body fluid (SBF) solution for 1 and 4 days. Sample morphology and chemistry were characterized via scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), respectively. The cytocompatibility was studied using human osteosarcoma (HOS) cell cultures and the direct contact assay. Mechanical characterization did not show significant differences in the compressive strength of the blends compared to pure cement controls. The bioactivity test revealed that the pastes induced surface precipitation of calcium phosphate (CaP) when exposed to the SBF solution (as confirmed by SEM and EDS). Non-carbonated pastes induced early CaP precipitation. Cytocompatibility experiments showed that the carbonated blends allowed adequate cell growth. Non-carbonated blends presented a highly cytotoxic behavior. The introduction of Metakaolin did not affect the cytocompatibility of the samples. These results show that Portland cement and Portland cement-Metakaolin blends could present suitable characteristics for applications as scaffolding materials in load bearing bone tissue engineering.



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1. Ministry, A.S., Mikos, A.G., Tissue engineering strategies for bone regeneration. Adv. Biochem Engin/Biotechnol, 2005. 94: p. 1
2. Salgado, A.J., Coutinho, O.P., Reis, R.L., Bone Tissue Engineering: State of the Art and Future Trends. Macromolecular Bioscience, 2004. 4: p. 776
3. Temenoff, J.S., Mikos, A.G., Review: tissue engineering for regeneration of articular cartilage. Biomaterials, 2000. 21: p. 2405
4. Kokubo, T., Takadama, H., How useful is SBF in predicting in vivo bone bioactivity?. Biomaterials, 2006. 27: p.2907
5. Kokubo, T., Kim, H.M., Kawashita, M., Novel bioactive materials with different mechanical properties. Biomaterials, 2003. 24: p. 2161
6. Friedman, C.D., Costantino, P.D., Takagi, S., Chow, L.C., Bone sourceTM hydroxyapatite cement: a novel biomaterial for craniofacial skeletal tissue engineering and reconstruction. J Biomed Mater Res (Appl Biomater), 1998. 43: p. 428
7. Adams, C.S., Mansfield, K., Perlot, R.L., Shapiro, I.M., Matrix Regulation of Skeletal Cell Apoptosis. J. Biol. Chem., 2001. 276: p. 20316
8. Kim, H.M., Kishimoto, K., Miyaji, F., Kokubo, T., Yao, T., Suetsugu, Y., Tanaka, J., Nakamura, T., Composition and structure of the apatite formed on PET substrates in SBF modified with various ionic activity products. J Biomed Mater Res, 1999. 46: p. 228
9. Rezwan, K., Chen, Q.Z., Blaker, J.J., Boccaccini, A.R., Biodegradable and bioactive polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials, 2006. 27: p.3413
10. Aïtcin, P.C., Cements of yesterday and today Concrete of tomorrow. Cement and Concrete Research, 2000. 30: 1349
11. Sabir, B.B., Wild, S., Bai, J., Metakaolin and calcined clays as pozzolans for concrete: a review. Cement & Concrete Composites, 2001. 23: p. 441
12. Sarkar, N.K., Caicedo, R., Ritwik, P., Moiseyeva, R., Kawashima, I., Physicochemical basis of the biological properties of mineral trioxide aggregate. J Endod, 2005. 31: p.97
13. Tay, F.R., Pashley, D.H., Guided tissue remineralization of partially demineralized human dentine. Biomaterials, 2007. 29: p.1127
14. Cultrone, G., Sebastian, E., Ortega Huertas, M., Forced and natural carboation of lime-based mortars with and without additives: mineralogical and textural changes. Cement and Concrete Research, 2005. 35: p. 2278
15. SJ, Northup, JN, Cammack. Mammalian cell culture models. En: Handbook of biomaterial evaluation: scientific, technical, and clinical testing of implant materials. 2 ed, Ann Arbor, Taylor & Francis, 1999, 329
16. Gallego, D., Higuita, N., Garcia, F., Ferrell, N., Hansford, D.J., Bioactive coatings on Portland cement substrates: Surface precipitation of apatite-like crystals. Materials Science and Engineering C, 2008. 28: p. 347
17. Huang, F.M., Tai, K.W., Chou, M.Y., Chang, Y.C., Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. International Endodontic Journal, 2002. 35: p. 153


Biocompatibility, Bioactivity and Mechanical Properties of Portland Cement and Portland Cement-Metakaolin Blends for Bone Tissue Engineering Applications

  • Daniel Gallego (a1), Natalia Higuita (a2), Felipe Garcia (a3), Olga M. Posada (a4), Luis E. Lopez (a5), Alan S. Litsky (a6) and Derek J. Hansford (a7)...


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