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Bilayer Composite Hydrogels for Corneal Prostheses

  • Edward Perez (a1), Linda G. Cima (a1), David Miller (a2) and Edward W. Merrill (a1)

Abstract

A two-layer composite material composed of a thin-layer of corneal tissue and a synthetic polyethylene oxide (PEO) hydrogel is described. The material is designed to provide a suitable substrate for corneal epithelial cell growth while maintaining the desirable characteristics of hydrogels, i.e. clarity, flexibility, and ability to allow diffusive flow of nutrients. The gels are synthesized via electron irradiation induced crosslinking of an aqueous solution of PEO onto a thin layer of collagenous tissue substrate. light microscopic studies indicate that the interface between the corneal tissue and PEO gel appears well adherent with no gaps in the interface. SEM studies of the material surface show an architecture similar to that of normal corneal tissue. Surface analytical techniques were used to identify amino-acids covalently bound to the gel at the gel/collagen interface after the proteinaceous material was removed. ESCA survey scans identified the presence of nitrogen on exposed gel/collagen interfaces and amino acid labelling confirmed the presence of amino acids. ATR-IR studies identifed increased absorption for the gel collagen interfaces at 1640 cm− 1 and 1540 cm−1 indicative of bound amino acids.

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1. Trinkhaus-Randall, V et al: The Developement of a Biopolymeric Keratoprosthetic Material. Investigate Ophthalmology and Visual Science; Vol 29, No 3 (1988) pp 393–400
2. Sipehia, R, Garphinkle, A, Jackson, W B, Chang TMS: Towards and Artificial Cornea. Biomat., Art Cell, Art Organs; 18(5) pp. 643655 (1990)
3. Thompson, K P: Current Status of Synthetic Epikeratoplasty. Refractive and Corneal Surgery; Vol.7 (1991) pp240248
4. Dole, M: The Radiation Chemistry of Macromolecules. Academic Press, New York (1972)4.
5. Shultz, A R: Radiation and Crosslinking by Radiation, in Fettes EM (ed.), Chemical Reactions of Polymers. Interscience, New York (1964)
6. Peiffer, R L, Werblin, T P, Fryczkowski AW: Pathology of Corneal Hydrogel Alloplastic Implants. Ophthalmology Aug 1985,92 (9) p. 1294–1304
7. Merrill, E W, Salzman, E W: Polyethylene Oxide as a Biomaterial. Amer Soc for Art Int Organs, Vol6, No 2 April/June (1983) p. 6064
8. Merrill, E W, et al.: Nonadsorptive Hydrogels for Blood Contact. Progress in Artificial Organs, ISAO Press, Cleveland (1986) pp. 909912
9. Maurice, D: The Cornea and Sclera. in The Eye, Vol 1B, Davson, H, editor. Florida, 1984, Academic Press, pp 1158
10. Geggel, H S, Friend, J, and Throft, R A: Collagen Gels for for Ocular Surface. Reports Invesigative Ophthalmology and Visual Science; 26: 901905 (1985)
11. Thompson, K P, Hanna, K D, and Gravagna, P. et al. Synthetic Collagen IV lenticules as a biomaterial for epikeratoplasty. ARVO Abstracts. Invest Ophthalmol Vis Sci. 1990; 31 (suppl):301
12. Azar, D T, Spurr-Michaud, S J, Tisdale, A S, Moore, M B, Gipson, I K: Reassembly of the Corneal Epithelial Adhesion Structures Following Human Epikeratoplasty. Archives of Ophthalmology Vol 109, Sept 1991 pp 12791284
13. Weetal, H H (editor), Immobilizing Enzymes, Antigen, Antibodies and Peptides: Preparation and Characterization. Markel Dekker Inc. New York (1975)
14. King, J K: Deciphering the Rules of Protein Folding. Chemical and Engineering News; 67(10) pp 3254 (1989)
15. Pietrucha, K, Kroh, J: Radiation Crosslinking of Poly(butyl acrylate) During Polymerization and Grafted Copolymerization with Cr(III) Crosslinked Collagen. Radiation Physics Chem 28(9) pp 373376 (1986)
16. Udenfriend, S, et al.: Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines. Science Vol 178, Nov 24, 1972
17. Komai, Y, Ushiki, T: The Three-Dimensional Organization of the Collagen Fibrils in the Human Cornea and Sclera. Invest Ophthal Vis Sci 2258, 1991
18. Yannas, I V: Collagen and Gelatin in the Solid State. J Macrolmolecular Sci.- Revs. Macromol Chem., C 7(1) (1972) p. 49104 18.
19. Vandeberg JT (editor): An Infrared Spectroscopy Atlas for the Coating Industry. Federation of the Society for Coating Technologies, Philadelphia PA (1980)
20. Dennison, K A: Radiation Crosslinked Poly(ethylene oxide) Hydrogel Membranes. PhD Thesis, MIT, (1986)
21. Silverstein, R M Clayton, Bassler, G, Merill, T C: Spectrometric Identification of Organic Compound, 4th Ed. John Wiley and Sons, Inc. New York (1981)
22. Kirkham, S M, Dangel, M F: The Keratoprosthesis: Improved Biocompatibility through Design and Surface Modification. Abstracts - 17th Cornea Research Conference, Boston (1991)
23. Okamura, S and Hino, T: Silicone Rubber Coated with Collagen. US Patent No 3, 955, 012 (May 4, 1976)
24. Brightbill, F S, Corneal Surgery: Theory, Tissue, and Technique. The C.V. Mosby Company St Louis, Missouri (1986)

Bilayer Composite Hydrogels for Corneal Prostheses

  • Edward Perez (a1), Linda G. Cima (a1), David Miller (a2) and Edward W. Merrill (a1)

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