Skip to main content Accessibility help
×
Home

Assessment of marine biofilm attachment and growth for antifouling surfaces under static and controlled hydrodynamic conditions

  • Maria Salta (a1), Julian A. Wharton (a1), Paul Stoodley (a1), Robert J.K. Wood (a1) and Keith R. Stokes (a1) (a2)...

Abstract

This investigation has assessed natural product antifouling performance of an isolated compound from a terrestrial source against marine biofilm forming bacteria, Cobetia marina and Marinobacter hydrocarbonoclasticus. Novel bioassay protocols using the hydrodynamic system and its well plate microfluidics capability were developed to test the in situ antifouling efficacy of the natural product against biofilm attachment under two shear stresses (0.07 and 0.3 Pa). The hydrodynamic results allowed for the first time the direct observation of the natural product influence on newly attached marine biofilms and the evolution of the antifouling affect with time. Biofilm attachment behaviour appeared to be markedly different in the presence of the natural product, illustrated by limited cluster and extracellular polymeric substance formation which suggests an interference of the bacterial attachment mechanisms. Ultimately, this is fundamental in developing greater understanding of the biofilm kinetics. These observations were confirmed using epifluoresence and confocal microscopy, with the additional corroborative data on bacterial cell integrity using the LIVE / DEAD nucleic acid kit.

Copyright

References

Hide All
1. Salta, M., Wharton, J.A., Stoodley, P., Dennington, S., Goodes, L., Werwinski, S., Mart, U., Wood, R.J.K., Stokes, K.R., Special Issue on Green Tribology – Designing biomimetic antifouling surfaces, Phil Trans A Royal Society, 368, 47294754 (2010).10.1098/rsta.2010.0195
2. Maximilien, R., De Nys, R., Holmstrom, C., Gram, L., Kjelleberg, S., Steinberg, P., Bacterial fouling is regulated by secondary metabolites from the red alga Delisea pulchra, Aquat. Microbial. Ecol. 15, 233246 (1998).10.3354/ame015233
3. Ekblad, T., Bergstrom, G., Ederth, T., Conlan, S., Mutton, R., Clare, A., Wang, S., Liu, Y., Zhao, Q., D’Souza, F., Donnelly, G., Willemsen, P., Pettitt, M., Callow, M., Callow, J., Liedberg, B., Poly (ethylene glycol)-containing hydrogel surfaces for antifouling applications in marine and freshwater environments, Biomacromolecules 9, 27752783 (2008).10.1021/bm800547m
4. Akesso, L., Pettitt, M., Callow, J., Callow, M., Stallard, J., Teer, D., Liu, C., Wang, S., Zhao, Q., Willemsen, P., The potential of nano-structured silicon oxide type coatings deposited by PACVD for control of aquatic biofouling. Biofouling, 25, 5567 (2009).10.1080/08927010802444275
5. D’Souza, F., Bruin, A., Biersteker, R., Donnelly, G., Klijnstra, J., Rentrop, C., Willemsen, P., Bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials, J. Indust. Microbiol. Biotechnol. 37, 363370 (2010).10.1007/s10295-009-0681-1
6. Benoit, M.R., Conant, C.G., Ionescu-Zanetti, C., Schwartz, M. and Matin, A., New Device for High-Throughput Viability Screening of Flow Biofilms, Appl. Environ. Microbiol. 76, 41364142 (2010).10.1128/AEM.03065-09
7. O’Brien, P., Molecular mechanisms of quinone cytocity, Chemico-Biological Interactions, 80, 141 (1991).10.1016/0009-2797(91)90029-7
8. Rieu, A., Briandet, R., Habimana, O., Garmyn, D., Guzzo, J., Piveteau, P., Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains, Appl. Environ. Microbiol. 74, 44914497 (2008).10.1128/AEM.00255-08
9. Beyenal, H., Lewandowski, Z., Internal and external mass transfer in biofilms grown at various flow velocities, Biotechnology Progress, 18, 5561 (2002).10.1021/bp010129s

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed