Skip to main content Accessibility help
×
Home
Hostname: page-component-559fc8cf4f-55wx7 Total loading time: 0.224 Render date: 2021-03-05T05:12:29.428Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Site-Specific Attachment of Gold Nanoparticles to DNA Templates

Published online by Cambridge University Press:  17 March 2011

Karen A. Stevenson
Affiliation:
Thomas Thundat Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Govindarajan Muralidharan
Affiliation:
Thomas Thundat Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Leon Maya
Affiliation:
Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Jack C. Wells
Affiliation:
Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Jacob Barhen
Affiliation:
Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Get access

Abstract

DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

Type
Research Article
Copyright
Copyright © Materials Research Society 2001

Access options

Get access to the full version of this content by using one of the access options below.

References

1. Chen, J., Seeman, N. C. Nature, 350, 631 (1991).CrossRefGoogle Scholar
2. Qi, J., Li, X., Yang, X., Seeman, N. C. J. Am. Chem. Soc., 118, 6121 (1996).CrossRefGoogle Scholar
3. Zhang, Y., Seeman, N.C. J. Am. Chem. Soc., 116, 1661 (1994).CrossRefGoogle Scholar
4. Li, X., Yang, X., Qi, J., Seeman, N. C. J. Am. Chem. Soc., 118, 6131 (1996).CrossRefGoogle Scholar
5. Winfree, E., Liu, F., Wenzler, L. A., Seeman, N. C. Nature, 394, 539 (1998).CrossRefGoogle Scholar
6. Mueller, J. E., Du, S. M., Seeman, N. C. J. Am. Chem. Soc., 113, 6306 (1991).CrossRefGoogle Scholar
7. Du, S. M., Seeman, N. C. Biopolymers, 34, 31 (1994).CrossRefGoogle Scholar
8. Mirkin, C. A., Letsinger, R. L., Mucic, R. C., Storhoff, J. J. Nature, 382, 607 (1996).CrossRefGoogle Scholar
9. Mitchell, G. P., Mirkin, C. A., Letsinger, R. L. J. Am. Chem. Soc., 121, 8122 (1999).CrossRefGoogle Scholar
10. Mirkin, C. A. Inorg. Chem., 39, 2258 (2000).CrossRefGoogle Scholar
11. Loweth, C. J., Caldwell, W. B., Peng, X., Alivisatos, A. P., Schultz, P. G. Angew. Chem. Int. Ed. 38, 1808 (1999).3.0.CO;2-C>CrossRefGoogle Scholar
12. Alivisatos, A. P., Johnsson, K. P., Peng, X., Wilson, T. E., Loweth, C. J., Bruchez, M. P. Jr., Schultz, P. G. Nature, 382, 609 (1996).CrossRefGoogle Scholar
13. Niemeyer, C. M., Burger, W., Peplies, J. Angew. Chem. Int. Ed., 37, 2265 (1998).3.0.CO;2-F>CrossRefGoogle Scholar
14. Cassell, A. M., Scrivens, W. A., Tour, J. M. Angew. Chem. Int. Ed., 37, 1528 (1998).3.0.CO;2-Q>CrossRefGoogle Scholar
15. Maya, L., Muralidharan, G., Thundat, T. G., Kenik, E. A. Langmuir, 16, 9151 (2000).CrossRefGoogle Scholar

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 10 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 5th March 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Site-Specific Attachment of Gold Nanoparticles to DNA Templates
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Site-Specific Attachment of Gold Nanoparticles to DNA Templates
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Site-Specific Attachment of Gold Nanoparticles to DNA Templates
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *