Hostname: page-component-cc8bf7c57-8cnds Total loading time: 0 Render date: 2024-12-12T05:49:40.210Z Has data issue: false hasContentIssue false
  • English
  • Français

Selective Killing of Ovarian Cancer Cells through Induction of Apoptosis by a Nonequilibrium Atmospheric Pressure Plasma

Published online by Cambridge University Press:  18 May 2012

Hiromasa Tanaka ,
Sachiko Iseki ,
Kae Nakamura ,
Moemi Hayashi ,
Hiroki Kondo ,
Hiroaki Kajiyama ,
Hiroyuki Kano ,
Fumitaka Kikkawa  and
Masaru Hori
Hiromasa Tanaka
Affiliation:
Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
Sachiko Iseki
Affiliation:
Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
Kae Nakamura
Affiliation:
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan
Moemi Hayashi
Affiliation:
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan
Hiroki Kondo
Affiliation:
Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
Hiroaki Kajiyama
Affiliation:
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan
Hiroyuki Kano
Affiliation:
NU Eco-Engineering Co., Ltd., 1237-87 Umazutsumi, Kurozasa-cho, Miyoshi-shi, Nishikamo-gun, Aichi 470-0201, Japan
Fumitaka Kikkawa
Affiliation:
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan
Masaru Hori
Affiliation:
Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
Get access

Abstract

Two independent ovarian cancer cell lines and fibroblast controls were treated with nonequilibrium atmospheric pressure plasma (NEAPP). Most ovarian cancer cells were detached from the culture dish by continuous plasma treatment to a single spot on the dish. Next, the plasma source was applied over the whole dish using a robot arm. In vitro cell proliferation assays showed that plasma treatments significantly decreased proliferation rates of ovarian cancer cells compared to fibroblast cells. FACS and Western blot analysis showed that plasma treatment of ovarian cancer cells induced apoptosis. NEAPP could be a promising tool for therapy for ovarian cancers.

Keywords

biomedicalbiologicalelectrical properties
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1469: Symposium WW – Plasma Processing and Diagnostics for Life Sciences , 2012 , mrss12-1469-ww02-04
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Kikkawa, F., Nawa, A., Kajiyama, H., Shibata, K., Ino, K., Nomura, S., Clinical characteristics and prognosis of mucinous tumors of the ovary, Gynecol. Oncol. 103, 171 (2006)10.1016/j.ygyno.2006.02.015Google Scholar
2. Lambert, H.E., Gregory, W.M., Nelstrop, A.E., Rustin, G.J., Long-term survival in 463 women treated with platinum analogs for advanced epithelial carcinoma of the ovary: life expectancy compared to women of an age-matched normal population, Int. J. Gynecol. Cancer 14, 772 (2004)10.1136/ijgc-00009577-200409000-00006Google Scholar
3. Ott, I., Gust, R., Non platinum metal complexes as anti-cancer drugs, Arch. Pharm. Chem. Life Sci. 340, 117 (2007)10.1002/ardp.200600151Google Scholar
4. Fridman, G., Friedman, G., Gutsol, A., Shekhter, A. B., Vasilets, V. N., and Fridman, A., Plasma Process Polym. 5, 503 (2008)10.1002/ppap.200700154Google Scholar
5. Kong, M. G., Kroesen, G., Morfill, G., Nosenko, T., Shimizu, T., Dijk, J. v., and Zimmermann, J. L., New J. Phys. 11 115012 (2009)10.1088/1367-2630/11/11/115012Google Scholar
6. Stoffels, E., Kieft, I. E. and Sladek, R. E. J., J. Phys. D: Appl. Phys. 36 2908 (2003)10.1088/0022-3727/36/23/007Google Scholar
7. Fridman, G., Shereshevsky, A., Jost, M.M., Brooks, A.D., Fridman, A., Gutsol, A., Vasilets, V., and Friedman, G., Plasma Chemistry and Plasma Processing 27 163 (2007)10.1007/s11090-007-9048-4Google Scholar
8. Lee, H.J., Shon, C.H., Kim, Y. S., Kim, S., Kim, G.C., and Kong, M.G., New J. Phys. 11 115026 (2009)10.1088/1367-2630/11/11/115026Google Scholar
9. Gweon, B., Kim, D.B., Kim, D., Kim, H., Jung, H., Shin, J.H., and Choe, W., Appl. Phys. Lett. 99 063701 (2011)10.1063/1.3622631Google Scholar
10. Iwasaki, M., Inui, H., Matsudaira, Y., Kano, H., Yoshida, N., Ito, M., and Hori, M., Appl. Phys. Lett. 92 081503 (2008)10.1063/1.2885084Google Scholar
11. Jia, F., Sumi, N., Ishikawa, K., Kano, H., Inui, H., Kularatne, J., Takeda, K., Kondo, H., Sekine, M., Kono, A., and Hori, M., Appl. Phys. Express 4 026101 (2011)10.1143/APEX.4.026101Google Scholar
12. Iseki, S., Ohta, T., Aomatsu, A., Ito, M., Kano, H., Higashijima, Y., and Hori, M., Appl. Phys. Lett. 96 153704 (2010)10.1063/1.3399265Google Scholar