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Influence of DSS-8 on the remineralisation of Dentine

Published online by Cambridge University Press:  31 January 2011

Chia-Chan Hsu
Affiliation:
richardhsu@ucla.edu, University of California Los Angeles, Materials Science and Engineering, Los Angeles, California, United States
Elizabeth Marie Hagerman
Affiliation:
sodium0303@hotmail.com, University of California Los Angeles, Bioengineering, Los Angeles, California, United States
Hsiu-Ying Chung
Affiliation:
richardhsu0303@yahoo.com, Feng Chia University, Materials Science and Engineering, Taichung, Taiwan, Taiwan, Province of China
Wenyuan Shi
Affiliation:
sodium0303@yahoo.com.tw, University of California, Los Angeles, School of Dentistry, Los Angeles, California, United States
Jenn-Ming Yang
Affiliation:
chiachan@seas.ucla.edu, United States
Ben Wu
Affiliation:
pqucla@yahoo.com, United States
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Abstract

Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in dentin remineralization. Human dentin specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Dentin nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using grazing incidence X-ray diffraction, variable pressure scanning electron microscopy, and atomic force microscopy, respectively. Nanoindentation results show that DSS-8 remineralization effectively improves the mechanical and elastic properties of native dentin. Moreover, the hardness and elastic modulus for the DSS-8 treated dentin were significantly higher than surfaces remineralized without DSS-8.

Type
Research Article
Copyright
Copyright © Materials Research Society 2009

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