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Image Analysis Method to Count Silver-Stained Candida in Aids Patients' Subgingival Plaques

Published online by Cambridge University Press:  28 February 2011

Jacob S. Hanker
Affiliation:
University of North Carolina, School of Dentistry and School of Medicine, CB #7455, Chapel Hill, NC 27599-7455
Hinesh B. Patel
Affiliation:
Departments of Chemical Engineering and Biochemistry, North Carolina State University, Raleigh, NC 27965
Beverly L. Giammara
Affiliation:
University of Louisville, Analytical Electron Microscopy Laboratory, Graduate Programs and Research, 2301 S. Third St., Louisville, KY 40292
Juergen Von Der Heiden
Affiliation:
Olympus Corporation, Precision Instrument Division, 4 Nevada Drive, Lake Success, NY 11042
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Abstract

Computer-assisted microscopic image analysis of silverstained subgingival plaque smears was shown to be far more dependable and reproducible than subjective scoring or rating by visual inspection to estimate the amounts of Candida 4ibicans(CA) present. High CA counts in subgingival plaques from atypical periodontal diseased sites could suggest that an individual be tested or monitored further for AIDS. This is especially the case if the individual is in a high risk group or if the CA count cannot be ascribed to immunosuppression resulting from diabetes, corticosteroid or broad-spectrum antibiotic therapy, cancer chemotherapy or blood transfusion for hemophilia. In many such situations this simple, rapid procedure may be useful in screening for AIDS when serologic procedures for the HIV virus may be equivocal.

Type
Research Article
Copyright
Copyright © Materials Research Society 1991

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References

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Image Analysis Method to Count Silver-Stained Candida in Aids Patients' Subgingival Plaques
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