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3D Micro-Tomographic imaging and Quantitative Morphometry for the Nondestructive Evaluation of Porous Biomaterials

Published online by Cambridge University Press:  10 February 2011

R. Müller ,
S. Matter ,
P. Neuenschwander ,
U. W. Suter  and
P. Rüegsegger
R. Müller
Affiliation:
Orthopedic Biomechanics Laboratory, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA. Institute for Biomedicai Engineering, University of Zürich and Swiss Federal Institute of Technology (ETH), Moussonstrasse 18, 8044 Zürich, Switzerland
S. Matter
Affiliation:
Institute of Polymers, Swiss Federal Institute of Technology (ETH), ETH Zentrum, 8092 Zurich, Switzerland.
P. Neuenschwander
Affiliation:
Institute of Polymers, Swiss Federal Institute of Technology (ETH), ETH Zentrum, 8092 Zurich, Switzerland.
U. W. Suter
Affiliation:
Institute of Polymers, Swiss Federal Institute of Technology (ETH), ETH Zentrum, 8092 Zurich, Switzerland.
P. Rüegsegger
Affiliation:
Institute for Biomedicai Engineering, University of Zürich and Swiss Federal Institute of Technology (ETH), Moussonstrasse 18, 8044 Zürich, Switzerland
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Abstract

Micro-computed tomography (μCT) is a new and emerging technique for the nondestructive assessment and analysis of the three-dimensional trabecular bone architecture. The applications of μCT with respect to the analysis of bone are manyfold. Nevertheless, it also holds high promise for the microstructural measurement and analysis of porous biomaterials. For the purpose of the study, a desk-top μCT providing a nominal isotropie resolution of 14 μm was used. Since the polymeric material has a very low X-ray absorption coefficient, the scaffolds were stained prior to measurement using a commercial X-ray contrast agent. This allowed not only to acquire important microstructural features of the diree-dimensional scaffold but also to compute standard structural indices such as BV/TV, BS/BV, Tb.N, Tb.Th, Tb.Sp and the degree of anisotropy (DA) using mean intercept length measurements. The preliminary results show that different types of scaffolds can be distinguished both qualitatively (visualization) and quantitatively (morphometry) provided an adequate X-ray staining technique is used. It can be concluded that, in the future, μCT may be of considerable help in basic as well as in applied research and development.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 461: Symposia BB – Morphological Control in Multiphase Polymer Mix… , 1996 , 217
Copyright
Copyright © Materials Research Society 1997

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References

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